It is thought that attenuation occurs especially in a laboratory setting because nonvirulent or weakly virulent mutants grow faster in laboratory media when there is no selective advantage to virulence.   pathogens lose virulence with age and survive longer in laboratory cultures.   there is selection for more virulent strains in the laboratory.   patients can be treated with drugs that induce attenuation.

Nutrition Now
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ISBN:9781337415408
Author:Brown
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Chapter32: The Multiple Dimensions Of Food Safety
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It is thought that attenuation occurs especially in a laboratory setting because

nonvirulent or weakly virulent mutants grow faster in laboratory media when there is no selective advantage to virulence.
 
pathogens lose virulence with age and survive longer in laboratory cultures.
 
there is selection for more virulent strains in the laboratory.
 
patients can be treated with drugs that induce attenuation.
 
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