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Introduction to Experiment 2: Protein Digestion by Pepsin In this experiment, we will look at for evidence of protein digestion using albumin, a protein, and Biuret reagent. Biuret reagent is used to test for the presence of proteins, or more specifically, peptide bonds. When long polypeptide chains are present the solution will turn violet, and when shorter polypeptide chains are present the solution will turn a lighter pink color. What is/are the end product(s) of protein digestion? 2. What is the purpose of the enzyme pepsin? 3. If protein digestion has occurred, will the solution be violet or pink (choose one)? Explain.

Introduction to Experiment 2: Protein Digestion by Pepsin In this experiment, we will look at for evidence of protein digestion using albumin, a protein, and Biuret reagent. Biuret reagent is used to test for the presence of proteins, or more specifically, peptide bonds. When long polypeptide chains are present the solution will turn violet, and when shorter polypeptide chains are present the solution will turn a lighter pink color. What is/are the end product(s) of protein digestion? 2. What is the purpose of the enzyme pepsin? 3. If protein digestion has occurred, will the solution be violet or pink (choose one)? Explain.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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### Introduction to Experiment 2: Protein Digestion by Pepsin In this experiment, we will look at evidence of protein digestion using albumin, a protein, and Biuret reagent. Biuret reagent is used to test for the presence of proteins, or more specifically, peptide bonds. When long polypeptide chains are present, the solution will turn violet, and when shorter polypeptide chains are present, the solution will turn a lighter pink color. 1. **What is/are the end product(s) of protein digestion?** (Write your answer here) 2. **What is the purpose of the enzyme pepsin?** (Write your answer here) 3. **If protein digestion has occurred, will the solution be violet or pink (choose one)? Explain.** (Write your answer here) #### Hypotheses/Predictions (See Methods section for details): 1. **What color do you expect the solution to turn when the P tubes are mixed with Biuret solution and why?** - P1: (Write your prediction) - P2: (Write your prediction) - P3: (Write your prediction) - P4: (Write your prediction) 2. **The least protein digestion (or none at all) will take place in tubes (P1, P2, P3, and/or P4)? Why?** (Write your prediction) 3. **What color will these tubes be after the Biuret test?** 4. **Of the remaining two tubes, tube _____ will have more protein digestion than tube _____. Why?** (Write your prediction) ### Introduction to Experiment 3: Lipid Digestion by Lipase In this experiment, we will investigate lipid digestion. Remember that lipids include fats, so the two terms are often used interchangeably. To do this, we will use litmus cream, which is a mixture of cream and litmus. Cream contains triglycerides (neutral fats). Litmus is a pH indicator. Purple indicates a neutral pH, pink indicates an acidic pH, and blue indicates a basic pH. 1. **How do bile salts act on a mixture of fat and water?** (Write your answer here) 2. **What is/are the end product(s) of fat digestion?** (Write your answer here) 3. **Is either of these end
Transcribed Image Text:### Introduction to Experiment 2: Protein Digestion by Pepsin In this experiment, we will look at evidence of protein digestion using albumin, a protein, and Biuret reagent. Biuret reagent is used to test for the presence of proteins, or more specifically, peptide bonds. When long polypeptide chains are present, the solution will turn violet, and when shorter polypeptide chains are present, the solution will turn a lighter pink color. 1. **What is/are the end product(s) of protein digestion?** (Write your answer here) 2. **What is the purpose of the enzyme pepsin?** (Write your answer here) 3. **If protein digestion has occurred, will the solution be violet or pink (choose one)? Explain.** (Write your answer here) #### Hypotheses/Predictions (See Methods section for details): 1. **What color do you expect the solution to turn when the P tubes are mixed with Biuret solution and why?** - P1: (Write your prediction) - P2: (Write your prediction) - P3: (Write your prediction) - P4: (Write your prediction) 2. **The least protein digestion (or none at all) will take place in tubes (P1, P2, P3, and/or P4)? Why?** (Write your prediction) 3. **What color will these tubes be after the Biuret test?** 4. **Of the remaining two tubes, tube _____ will have more protein digestion than tube _____. Why?** (Write your prediction) ### Introduction to Experiment 3: Lipid Digestion by Lipase In this experiment, we will investigate lipid digestion. Remember that lipids include fats, so the two terms are often used interchangeably. To do this, we will use litmus cream, which is a mixture of cream and litmus. Cream contains triglycerides (neutral fats). Litmus is a pH indicator. Purple indicates a neutral pH, pink indicates an acidic pH, and blue indicates a basic pH. 1. **How do bile salts act on a mixture of fat and water?** (Write your answer here) 2. **What is/are the end product(s) of fat digestion?** (Write your answer here) 3. **Is either of these end
**Introduction to Experiment 1: Starch Digestion by Amylase** In this experiment, we will look for evidence of starch digestion by testing solutions for the presence of starch and sugars. When starch is present, the iodine in **Lugol's solution** turns the solution black, blue, or dark brown. If starch is not present, iodine will dye the solution a yellow color. **Benedict’s reagent** reacts with "reducing sugars," including glucose, resulting in a green, orange, or red color. If sugars are not present, Benedict’s reagent remains a light blue color. 1. **What is/are the end product(s) of starch digestion?** _______________________________________________ 2. **What is the name of the enzyme that digests starch (amylase)?** _______________________________________________ **Hypotheses/Predictions (See Methods section for details):** 1. **When mixed with Lugol's solution, test tube A1 (amylase only) will have what color and why?** _______________________________________________ 2. **When mixed with Benedict’s solution, test tube A1 (amylase only) will have what color and why?** _______________________________________________ 3. **When mixed with Lugol's solution, test tube A2 (starch only) will have what color and why?** _______________________________________________ 4. **When mixed with Benedict’s solution, test tube A2 (starch only) will have what color and why?** _______________________________________________ 5. **When mixed with Lugol's solution, test tube A3 (glucose only) will have what color and why?** _______________________________________________ 6. **When mixed with Benedict’s solution, test tube A3 (glucose only) will have what color and why?** _______________________________________________ 7. **When mixed with Lugol's solution, test tube A4 (amylase and starch) will have what color and why?** _______________________________________________ 8. **When mixed with Benedict’s solution, test tube A4 (amylase and starch) will have what color and why?** _______________________________________________
Transcribed Image Text:**Introduction to Experiment 1: Starch Digestion by Amylase** In this experiment, we will look for evidence of starch digestion by testing solutions for the presence of starch and sugars. When starch is present, the iodine in **Lugol's solution** turns the solution black, blue, or dark brown. If starch is not present, iodine will dye the solution a yellow color. **Benedict’s reagent** reacts with "reducing sugars," including glucose, resulting in a green, orange, or red color. If sugars are not present, Benedict’s reagent remains a light blue color. 1. **What is/are the end product(s) of starch digestion?** _______________________________________________ 2. **What is the name of the enzyme that digests starch (amylase)?** _______________________________________________ **Hypotheses/Predictions (See Methods section for details):** 1. **When mixed with Lugol's solution, test tube A1 (amylase only) will have what color and why?** _______________________________________________ 2. **When mixed with Benedict’s solution, test tube A1 (amylase only) will have what color and why?** _______________________________________________ 3. **When mixed with Lugol's solution, test tube A2 (starch only) will have what color and why?** _______________________________________________ 4. **When mixed with Benedict’s solution, test tube A2 (starch only) will have what color and why?** _______________________________________________ 5. **When mixed with Lugol's solution, test tube A3 (glucose only) will have what color and why?** _______________________________________________ 6. **When mixed with Benedict’s solution, test tube A3 (glucose only) will have what color and why?** _______________________________________________ 7. **When mixed with Lugol's solution, test tube A4 (amylase and starch) will have what color and why?** _______________________________________________ 8. **When mixed with Benedict’s solution, test tube A4 (amylase and starch) will have what color and why?** _______________________________________________
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