Individuals infected with herpes simplex virus (HSV) mount protective antibody responses directed against surface glycoproteins of the virus. These antibodies are critical to viral clearance. The act to neutralise any virus trying to infect a person's cells as well as activating the classical pathway of complement and cytotoxic cell- mediated killing of infected target cells. Having a deficiency in the complement component C3 greatly impairs a person's ability to control HSV infections. To understand this further, you will investigate this in the lab. Using C3-deficient mice, you infect them with HSV at day 0 and then a second time 4 weeks later. Anti-HSV IgG Ab (µg/ml) 10³. 102. 10¹. 10⁰. 1 2 3 4 5 Primary infection Secondary infection Figure 1 6 Wild-type C3 7 8 Weeks post- vaccination A hypothesis is a tentative answer to a scientific question and is an important part of practical experiments. A testable hypothesis is statement that can be proved or disproved as a result of experimentation. You perform an ELISA to measure the antibody response of the C3-deficient and normal mice (referred to as wild-type - WT) infected with HSV. The results are shown in Figure 1. Q2. Looking at Figure 1, describe the results of the ELISA.
Individuals infected with herpes simplex virus (HSV) mount protective antibody responses directed against surface glycoproteins of the virus. These antibodies are critical to viral clearance. The act to neutralise any virus trying to infect a person's cells as well as activating the classical pathway of complement and cytotoxic cell- mediated killing of infected target cells. Having a deficiency in the complement component C3 greatly impairs a person's ability to control HSV infections. To understand this further, you will investigate this in the lab. Using C3-deficient mice, you infect them with HSV at day 0 and then a second time 4 weeks later. Anti-HSV IgG Ab (µg/ml) 10³. 102. 10¹. 10⁰. 1 2 3 4 5 Primary infection Secondary infection Figure 1 6 Wild-type C3 7 8 Weeks post- vaccination A hypothesis is a tentative answer to a scientific question and is an important part of practical experiments. A testable hypothesis is statement that can be proved or disproved as a result of experimentation. You perform an ELISA to measure the antibody response of the C3-deficient and normal mice (referred to as wild-type - WT) infected with HSV. The results are shown in Figure 1. Q2. Looking at Figure 1, describe the results of the ELISA.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:F
4
This paper will test your knowledge and ability to gather, analyse and interpret data from
experimental immunology.
Laboratory Scenario:
Individuals infected with herpes simplex virus (HSV) mount protective antibody responses
directed against surface glycoproteins of the virus.
These antibodies are critical to viral clearance. The act to neutralise any virus trying to infect
a person's cells as well as activating the classical pathway of complement and cytotoxic cell-
mediated killing of infected target cells. Having a deficiency in the complement component
C3 greatly impairs a person's ability to control HSV infections.
To understand this further, you will investigate this in the lab. Using C3-deficient mice, you
infect them with HSV at day 0 and then a second time 4 weeks later.
%
5
D
F5
Anti-HSV IgG Ab (μg/ml)
<C
6
10³.
10².
10¹
10⁰-
1 2 3 4
Primary
infection
5 6
A hypothesis is a tentative answer to a scientific question and is an important part of practical
experiments. A testable hypothesis is statement that can be proved or disproved as a result
of experimentation.
You perform an ELISA to measure the antibody response of the C3-deficient and normal mice
(referred to as wild-type-WT) infected with HSV. The results are shown in Figure 1.
Q2. Looking at Figure 1, describe the results of the ELISA.
F6
Secondary
infection
Figure 1
Wild-type
&
7
C3
T
7
8
Weeks
post-
vaccination
F7
8
DII
F8
P
F9
Focus
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