Individual proteins of known subunit molecular weight and the pyrophosphatase with a native molecular weight of39,000 dals were denatured in a buffer containing both SDS and B-mercaptoethanol. A sample of each protein waselectrophoretically separated by SDS-polyacrylamide gel electrophoresis. The marker dye migrated 12 cm. Staining thegel for protein revealed the following:ITDistance Migrated (cm)MW69,00060,00043,00029,000ProteinSerum albumin1.6catalase2.23.5ovalbumincarbonic anhydrasemyoglobin5.217,0007.4The pyrophosphatase migrated 6.7 cm. However, when the B-mercaptoethanol was omitted, the pyrophosphatasemigrated 3.8 cm. Determine the subunits molecular weight and comment on the quaternary structure of the enzyme.

The sample one can be defined as, The Pyrophosphatase, SDS and β-Mercaptoethanol has the migration…

Answered: Individual proteins of known subunit…

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