ou have been tasked with scaling up the capture chromatography step that you designed in lab for the GFPuv process. Your large-scale column will be used to load approximately 100 L of the same clarified lysate. So that you all use the same values in your calculation, assume that you loaded 7.5 CV of clarified lysate to a 45 mL column during development studies. The concentration of GFP in the clarified lysate is 1.89 mg GFP/mL lysate. What is the height of the production column? What diameter of column is required? What volumetric flow rate, in L/h, will you use at the larger scale?
ou have been tasked with scaling up the capture chromatography step that you designed in lab for the GFPuv process. Your large-scale column will be used to load approximately 100 L of the same clarified lysate. So that you all use the same values in your calculation, assume that you loaded 7.5 CV of clarified lysate to a 45 mL column during development studies. The concentration of GFP in the clarified lysate is 1.89 mg GFP/mL lysate. What is the height of the production column? What diameter of column is required? What volumetric flow rate, in L/h, will you use at the larger scale?
Chapter4: Mass Spectrometry: Part Two: Fragmentation And Structural Analysis
Section: Chapter Questions
Problem 9P
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You have been tasked with scaling up the capture chromatography step that you designed in lab for the GFPuv process. Your large-scale column will be used to load approximately 100 L of the same clarified lysate.
So that you all use the same values in your calculation, assume that you loaded 7.5 CV of clarified lysate to a 45 mL column during development studies. The concentration of GFP in the clarified lysate is 1.89 mg GFP/mL lysate.
- What is the height of the production column?
- What diameter of column is required?
- What volumetric flow rate, in L/h, will you use at the larger scale?
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