In the absence of actin, myosin will hydrolyze ATP, but very slowly. Based on the mechanism shown in the figure, what could explain this slow rate? ΟΟ Without actin, the reversible ATP hydrolysis by myosin shifts in favor of ATP synthesis. Without actin, the attacking water molecule cannot easily reach the ATP bound to myosin. Without actin, myosin releases ADP and P; very slowly after hydrolyzing ATP. Without actin, myosin cannot undergo the conformational changes needed to hydrolyze ATP.

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