In microbiology, dilutions are critical for getting a countable number of microbes to grow on a petri dish. If you suspect your culture of bacteria has 50 x 106 cells per mL, what would you want the final dilution to be in order to end up with 50 colonies of bacteria on a petri dish? Express your answer as an exponent rounded to two decimal places. Eg. 10.01e-3, not 0.00101. Treat colonies of bacteria (cells / mL) the way you would treat concentration (Moles / L) The formula M1V1 = M2V2 can still be used. The final concentration is the number of colonies on the petri dish. Solve %3D for V2. A 0.00000001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 150 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. • Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.

In microbiology, dilutions are critical for getting a countable number of microbes to grow on a petri dish. If you suspect your culture of bacteria has 50 x 106 cells per mL, what would you want the final dilution to be in order to end up with 50 colonies of bacteria on a petri dish? Express your answer as an exponent rounded to two decimal places. Eg. 10.01e-3, not 0.00101. Treat colonies of bacteria (cells / mL) the way you would treat concentration (Moles / L) The formula M1V1 = M2V2 can still be used. The final concentration is the number of colonies on the petri dish. Solve %3D for V2. A 0.00000001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, 0.1 mL of solution is plated on solid media, and 150 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. • Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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