In lab, one of your classmates is confused because their calculations for bacteria in 1.0 gram of soil for each of their dilution plates is very similar! They comment, "I counted different numbers of colonies - why are they so close?" What will you tell them about their calculations?

Q: Nadia collected samples of water from Tasik Harapan, Universiti Sains Malaysia to isolate and…

A: The pH of a solution indicates how acidic or basic it is.

Q: One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies…

A: A colony-forming unit, or CFU, is a measurement of the concentration of microorganisms in a test…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

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Q: how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.

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Q: You have a culture that has 5 X103 cfu/ml of bacteria in it. You perform a serial dilution by adding…

A: In microbiology and bacteriology, the technique of serial dilution is a fundamental method used to…

Q: You have spread 0.1ml of a 1x10-8 diluted bacterial culture sample on a Petridish and counted 35…

A: Given  Volume plated = 0.1ml  Total dilution = 108  Number of colony = 35

Q: After treating the bacterium Examplicus expedientius with a chemical agent to test the effectiveness…

A: Answer.) :- The Decimal Reduction Time (DRT) is a measure used to evaluate the effectiveness of a…

Q: Each Escherichia coli cell weighs 5ng. How many cells are present in a colony that weighs 1mg? Write…

A: Escherichia coli is a Gram-negative bacteria. It is present as normal intestinal flora but can also…

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Q: ou've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture.…

A: Bacteria is the tiny cellular living structure which belong to the  Moneran kingdom. The bacteria…

Q: A microbiology student was given a mixed culture of two different gram positive bacteria species to…

A: In Microbiology, we grow bacteria on culture medium. Culture media contains specific mixture of…

Q: You prepared 10-7, 10-8, and 10-9 dilutions of a bacterial suspension in sterile saline. Then you…

A: Introduction There are different culturing techniques such as agar plates, broth culture, stab…

Q: Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 μL of bacteria…

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Q: Suppose you are to observe an unknown bacterial slide. In your initial observation, it appears to be…

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Q: Using a dilution series is a way to transfer the very small volumes of original culture necessary to…

A: Answer -To determine the volume of the original bacterial culture required to obtain a specific…

Q: Complete the following questions for your gram negative rod unknown organism (a) What test did you…

A: The gram-negative rod group is a diverse and extensive category of bacteria characterized by their…

Q: Imagine you made a single kirby bauer plate to determine if a given antibiotic were effective for…

A: Introduction Antibiotics, also called antibacterials, are drugs that kill or slow the growth of…

Q: 1 mL of a juice sample is taken and diluted in 9 mL culture medium, subsequently, this suspension is…

A: Petroff- Hausser chamber is a chamber used usually for sperm and bacterial count. Number of bacteria…

Q: Suppose your professor handed you a bottle of kombucha with 2.0 ml in it and told you to make a…

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Q: A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture…

A: A growth medium is intended to promote the growth of a variety of microorganisms or cells by the…

Q: ) = 80e−0.05t + 8(t + 1)−0.5. Her model says that C(t) is the number of coliform group bacteria…

A: If considering the equation C(t) = 80e−0.05t + 8(t + 1)−0.5  Time is zero initially then  C =…

Q: Each Escherichia coli cell weighs 5ng. How many cells are present in a colony that weighs 1mg?

A: E. coli is a rod-shaped, Gram-negative, facultative anaerobic coliform bacteria of the genus…

Q: A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine…

A: To keep the number of colonies on the plate within a certain range, serial dilutions of culture are…

Q: Why do we use the term CFU (colony forming unit) instead of reporting bacteria per ml? Explain.

A: The  bacterial species are responsible for causing several diseases within the human body. So, for…

Q: You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of  fecal…

A: The evaluation of fecal microscopic organisms in water could be a critical public well-being measure…

Q: A microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its…

A: Psychrophilic bacteria are cold loving bacteria that grows and reproduce at lower temperatures…

Q: You perform several tests on an unidentified bacterial species and get the results below. Based on…

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Q: You perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults:…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: You use a spectrophometer and estimate the concentration of bacteria in a urine sample to be 4.6 x…

A: dilution factor  =10^-6

Q: expen using disinfectant yielded the following results: Control (no disinfectant) - 220 colonies on…

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Q: Explain how to perform a serial dilution

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Q: According to these images and your own knowledge, can you make a biological drawing of bacteria in…

A: Yoghurt is a nutrient which is rich in lactobacillus (lactic acid bacteria) . Convert milk into…

Q: A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From…

A: Serial dilution is a process by which a substance such as a bacterial culture is diluted stepwise in…

Q: Citing the data from your results table tell which antibiotic is the most effective against this…

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Q: You perform a set of serial dilutions using 99-mL water blanks (successive 1-mL dilutions into…

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Q: You have several different media onto which you inoculated eight strains of yeast (A-H). The media…

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Q: MacConkey agar is often used to differentiate E. coli from other Gram-negative rods. The recipe for…

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Q: Streaking One drop of bacterial solution contains millions of cells. To identify a species,…

A: The purpose of streaking for isolation is to produce isolated colonies of an organism on an agar…

Q: Assume that you counted 67 plaques on a bacterial plate where 0.1ml of a 10-5 dilution of phage was…

A: A dilution: It can be defined as a method of addition of more solvent to a solution of a definite…

Q: A slant is inoculated with 100 E.coli cells. The slant is left at room temperature where the…

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Q: From a soup sample, 1 mL of sample is taken and diluted in 99 mL of soup medium. culture, later,…

A: The volume of a single square of Petroff-hausser chamber = 0.02 cube mm = 2×10-5 cm3 1 centimeter…

Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…

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Q: A stock solution of a bacterial strain is diluted 1/10000 to a total volume of 500ul. Then, 50ul of…

A: The mixing of the stock solution with the dilutant liquid is known as dilution. The ratio of stock…