In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar plate?

Q: What types of samples can be measured with Circular Dichroism? Can information be extracted?

A: Circulate dichroism is the difference in the absorption of left handed circularly polarised…

Q: What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and…

A: The dilution factor is a measure of how much a solution has been diluted or concentrated. It is…

Q: You have added 8 mL of Albuterol Sulfate Solution (5mg/mL) and 22 mL of normal saline to your…

A: Introduction:-  Albuterol sulfate solution is in concentration of 5mg/ml and total of 8ml is to…

Q: What is the principle of freezing of cell lines and primary cells.Describe the general protocol for…

A: Cell culture is a procedure in which cells (either animal or plant cells) are separated from an…

Q: What is the pore size of the membrane filter used in the membrane filter method for water analysis?

A: Microorganisms are small organism that cannot be seen by naked eyes. Infectious disease is caused by…

Q: In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was…

A: Coating buffer in ELISA is a mix of sodium carbonate and bicarbonate at ph 9.4. This gives charge to…

Q: Give two reasons as to why anyone would bother SYBRGreen (SG) staining samples that contain…

A: SYBR Green (SG) is an asymmetrical cyanine nucleic acid stain. It binds to DNA.

Q: Using one of the graphs obtained with the medium II, name the different phases of the bacterial…

A: Micro organisms are typically grown in a closed culture media. Nutrients are added according to the…

Q: Given: The used ocular objective while taking this image, has magnifying power of 6x The used…

A: Given : Stage micrometer used has graduation = 0.01mm Ten graduation on ocular micrometer coincides…

Q: Explain the expressions of primary culture, cell haH, and continuous cell haH. Explain the reason…

A: Answers :  Th cells in the primary culture are heterogeneous as they are freshly obtained from the…

Q: In a fed-batch culture operating with intermittent addition of lactose solution, values ofthe…

A: Note: As per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction:  A…

Q: Why would it be important for the Kirby-Bauer disc diffusion test to use a standard concentration of…

A: An antibiotic susceptibility test is the disk diffusion or agar diffusion test, or Kirby-Bauer test…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: Discuss the role of the addition of ammonium chloride potassium lysius buffer in splenocyte culture…

A: Any form of white blood cell that is found in the spleen or has been separated from splenic tissue…

Q: Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 μL of bacteria…

A: For calculating the unknown concentration of the tube we will be using the formula as mentioned…

Q: No zone of inhibition was seen on the Blood Agar Plate with the Optochin Test. How should the…

A: zone of inhibition: This is an area of media where bacteria are unable to grow, due to presence of a…

Q: Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features…

A: Staphylococcus aureus and Streptococcus pyogenes are gram positive bacteria that cause infections in…

Q: Can you please discuss the principles of using Motility Test Medium for the detection of bacterial…

A: Bacterial motility is usually demonstrated in a semi-solid agar medium. Motile bacteria ‘swarm' in…

Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…

A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: Compare and contrast the method for determining the minimum inhibitory concentration (MIC) with the…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: How does gel filtration work? What are the applications of analytical versus preparative versions…

A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: What medium is this? What is the result in tube at left? In second tube? What does it tell you about…

A: One stab towards the bottom of the SIM tube is used to inoculate it. If the microorganism is motile,…

Q: What are the difference in the movement of microorganisms under wet mount from hanging drop…

A: There are some plus point for both the techniques like the wet Mount and hanging drop method…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the…

A: Serial dilution is used mostly in studies involving cell cultures (microbiology or even cell culture…

Q: What is the effect of trypsin EDTA solution on monolayer cell culture preparation?

A: Trypsin is an enzyme which helps in digestion of proteins.In the small intestine,proteins are broken…

Q: You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the…

A: Estimation of the number of viable cells is important in microbiology protocols. It is estimated…

Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1)…

A: Aseptic technique is most commonly used in microbiology and biotechnology lab. It refers to the…

Q: A milk sample was tested for the presence of typhimurium. Using a 10 microliter calibrated loop, a…

A: Serial dilution is the technique to find the concentration of bacteria in a sample when there are…

Q: In autoclaving media and glassware, why do you need to maintain the temperature at 121 0C and…

A: Microbial culture is done in sterile environments. The media and glassware used in the process are…

Q: Voges Proskauer test Does color of the medium change to red after both reagents were added and…

A: Biochemical method of bacterial identification is a classical method that is used for the…

Q: Explain how to conduct a Kirby-Bauer disk diffusion test. Include all of the steps involved

A: INTRODUCTION Kirby - Bauer Disc diffusion method Kirby - bauer disc diffusion method is widely known…

Q: Distance of color diameter (mm) 20 15 KMNO, 0.1M - KMNO, 1.0M - 5. 15 45 09 75 Time (minutes) 06

A: * The molecules movement in  agarose gel is dependent on the  size  charge  *And pore size also…

Q: For the following agar media used, list their significant components and explain how they react.…

A: The sheep blood agar is a popular agar medium. It is a differential medium that includes important…

Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…

A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…

Q: Why should bacterial culture plates be incubated inverted (bottom up)?

A: Microbial isolation is a process done in microbiology lab in order to obtained microbe of interest…

Q: What is the most commonly used fixative in stool samples and why?

A: Fixative is a moist environment for transporting and preserving intestinal parasites in stool…

Q: laboratory? explain with diagram

A: The tissue culture is the general term for the removal of the cells, tissues or organs from an…

Q: Consider the sterilization of a sodium gluconate production medium in the holding section of a…

A: Answer :- Sterilization is a critical process in various industries, including food and…

Q: After an antimicrobial drugs test, the following results were obtained from a disk-diffusion test…

A: The antibiotic disc diffusion test is usually performed on an agar plate. The agar plate contains a…

Q: How is the information from a Kirby-Bauer disk diffusion test used for the recommendation of the…

A: Disclaimer: Kindly repost for other questions. As per the guidelines we are supposed to answer only…

Q: Can a P1000 micropipette be used to accurately pipet a glycerol solution? Why or why not? What about…

A: We must obtain accurate and exact measurements in order to achieve adequate and effective…

Q: To measure the culture temperature, why is the thermometer placed in a tube separate from the…

A: A microbe or microorganism is a microscopic organism that is too small to be seen with the naked…

Q: You just finished plating your electroporatio products on your YPD-Zeocin plate and you think you…

A: Electroporation is a technique used to introduce foreign DNA into cells by applying an electric…

Q: Develop a spreadsheet to predict as functions of time with concentration, volimme and permeate flows…

A: Introduction: The given flux equation depends on the concentration of the retentate, and other…

Q: How to calculate dilutions in standard plate count?  What is the microscopic counting chamber? How…

A: Serial dilutions are used to calculate the concentration of microorganisms.

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.