In a fluorescent microscope setup, you have a relatively dim sample. You have two objectives available, a 100x NA 1.2 oil objective and a 60x NA 0.72 air objective. Which would be better for your dim sample?
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In a fluorescent microscope setup, you have a relatively dim sample. You have two objectives available, a 100x NA 1.2 oil objective and a 60x NA 0.72 air objective. Which would be better for your dim sample?
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- I don't think my work for this problem is correct. The data is given in the images. There was no physical experiment doneThe Rayleigh criterion provides a convenient way to describe the theoretical resolution (e.g. an ability to distinguish objects ) of an optical system. The criterion states that two small bright sources of light can be resolved if the first d mìnimum of the image of one source point just coincides with of further apart then the first maximum of another (s Ho Your c below). A converging lens, 28.7 mm in diameter, is used to form images of distant objects. Considering the diffractio lens, what angular separation must two distant point objects have in order to satisfy Rayleigh's criterion? Assume t Here wavelength of the light from the distant objects is 469 nm. Provide your answer in millidegrees (mdeg). STE Exp complexity subscibers Answer: Choose... + Check Finishin Check cThree sheets of plastic have unknown indices of refraction. Sheet 1 is placed on top of sheet 2, and a laser beam is directed onto the sheets from above so that it strikes the interface at an angle of 26.6deg with the normal. The refracted beam in sheet 2 makes an angle of 31.4deg with the normal. The experiment is repeated with sheet 3 on top of sheet 2 and, with the same angle of incidence, the refracted beam makes an angle of 36.8deg with the normal. If the experiment is repeated again with sheet 1 on top of sheet 3, what is the expected angle of refraction in sheet 3? Assume the same angle of incidence.
- A worm is 3 mm in size and occupies 60% of the FOV. What is the diameter of the FOV at this unknown magnification?Impressionist painter Georges Seurat created paintings with an enormous number of dots of pure pigment, each of which was approximately 2.00 mm in diameter. The idea was to have colors such as red and green next to each other to form a scintillating canvas, such as in his masterpiece, A Sunday Afternoon on the Island of La Grande Jatte (figure below). Assume 1 = 420 nm and a pupil diameter of 4.90 mm. Beyond what distance would a viewer be unable to discern individual dots on the canvas? m SuperStock/SuperStockWhat is the smallest thing we can see? O smallest object that we can process with our eyes is limited to the size of the photoreceptor cells in the retina. In order for us to to distinguish any detail in an object, its image cannot be smaller cannot be smaller than a single retinal cell. Although the size depends on the type of cell (cone or rod), a diameter of of a few microns (mm) is common near the center of the eye. We should model the eye as a sphere 2.50 cm in diameter with a single slender lens in front and the retina behind, with photoreceptor cells 5.0 mm in diameter. (a) What is the smallest object object that you can perceive at a point near 25 cm? What angle is subtended by this object in the eye? Express your answer in units of minutes (1° 60 min) and compare it with the typical experimental value of about 1.0 min. (Note: there are other limitations, but we will ignore them here).