Imagine you want to want to make a phosphate buffer in the lab that resembles the bloods phosphate buffer. You start by mixing 0.40 M NaH2PO4 and 0.60 M Na2HPO4. H2PO4-  + H2O ⇌  HPO42-  + H+          pKa= 7.21    From the reaction above indicate the weak acid and the conjugate base involved in making this buffer.  Calculate the pH of the phosphate buffer solution? Provide your answer to 2 decimal places  If the pH calculated in b was the blood pH of an actual patient, would you say this patient’s blood pH is within normal range or are they suffering from alkalosis or acidosis?

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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For humans to maintain the pH of their blood close to 7.4, the body uses three buffer systems: carbonate, phosphate and proteins. If the pH of the blood goes below 6.8 or above 7.8, death may result.

Imagine you want to want to make a phosphate buffer in the lab that resembles the bloods phosphate buffer. You start by mixing 0.40 M NaH2PO4 and 0.60 M Na2HPO4.

H2PO4-  + H2O ⇌  HPO42-  + H+          pKa= 7.21   

  1. From the reaction above indicate the weak acid and the conjugate base involved in making this buffer. 
  2. Calculate the pH of the phosphate buffer solution? Provide your answer to 2 decimal places 
  3. If the pH calculated in b was the blood pH of an actual patient, would you say this patient’s blood pH is within normal range or are they suffering from alkalosis or acidosis? 
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