If you are going to prepare 100 ml each of the following two (2) buffers: • Buffer 1 will have a concentration of 0.2 M and a pH of 4.4 • Buffer 2 will have a concentration of 0.15 M and a pH of 8.9 The buffering systems available to you are • dihydrogen phosphate/ hydrogen phosphate pKa= 6.8 • Tris-HCl/ Tris base pKa = 8.1 • acetic acid/ acetate pKa =4.8 What buffering system would you use for buffer 1 and buffer 2? why?
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If you are going to prepare 100 ml each of the following two (2) buffers:
• Buffer 1 will have a concentration of 0.2 M and a pH of 4.4
• Buffer 2 will have a concentration of 0.15 M and a pH of 8.9
The buffering systems available to you are
• dihydrogen phosphate/ hydrogen phosphate pKa= 6.8
• Tris-HCl/ Tris base pKa = 8.1
• acetic acid/ acetate pKa =4.8
What buffering system would you use for buffer 1 and buffer 2? why?
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