If you perform a column chromatography based on the present TLC analysis, which compound is eluted firstly from the column? (Yellow or Brown one). What are the most used adsorbents (stationary phase) in a column chromatography analysis? Which fractions of the column chromatography analysis do you keep containing possible pure compound which are shown on the TLC analysis? 56789 10 11 12 13 14

If you perform a column chromatography based on the present TLC analysis, which compound is eluted firstly from the column? (Yellow or Brown one). What are the most used adsorbents (stationary phase) in a column chromatography analysis? Which fractions of the column chromatography analysis do you keep containing possible pure compound which are shown on the TLC analysis? 56789 10 11 12 13 14

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Answer the following questions.
a. Say you are trying to separate two compounds by column chromatography. You run a column and collect the eluent in five fractions. You analyze each fraction by TLC on the same plate (shown below). Based on the TLC plate, how effective was your separation? Consider the purity of each fraction. - - - - - - - - ----| 1 2 3 4 5
In the diagram of TLC for column chromatography, What does light faded looking spots (less or less product, etc.)? Which fractions contain the product? concentrated 1 2 3 4 14 26 15 27 16 28 17 29 5 6 7 8 18 19 30 31 32 20 33 9 21 10 22 11 23 12 13 24 25 34 35 36 37 38
Explain how Thin Layer Chromatography allows for the separation of the components of a mixture on a TLC plate? Please include these words: equilibrium, stationary and mobile phase, nonpolar and polar analytes, affinitity...which analytes (polar/nonpolar) have greater affinity for stationary or mobile phase?
What is stationary phase used is liquid chromatography? What compounds does it bind best and why?
A mixture of three compounds was chromatographed using a silica gel column with diethyl ether/hexanes (10:90) as the eluting solvent. Fractions were collected and TLC analysis of each fraction was performed, with the results shown here: A 3 4 7 8 Mixture before separation Which fractions should be combined to give pure samples of A, B and C?
enumerate the advantage of TLC methods of chromatography over column and paper chromatography.
Consider the following chromatographic data for the separation of methylparaben from ethylparaben in a sunscreen sample. Compound Methylparaben Retention time, tr (min). Width at base of peak, Wb (min) 3.2 4.2 0.55 0.60 Ethylparaben A compound not retained by the column in this analysis had a tm of 0.75 minutes. The column used was a C18 column with a mobile phase mixture of 75% methanol and 25% water. Calculate the number of theoretical plates for ethylparaben.
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What is the main difference between reverse phase HPLC and standard ion-exchange or gel filtration chromatography?