If energy-coupling between the reactions ADP ---> ATP and H-P --> H is direct, how many enzymes and how many steps are required, and how is coupling accomplished? 1. One enzyme, two steps; the enzyme binds ADP and H-P at the same time. 2. One enzyme, one step; the enzyme binds ADP and H-P at the same time. 3. Two enzymes, two steps; one enzyme binds ATP and one binds H at the same time. 4. One enzyme, one step; the enzyme binds ATP and H at the same time. 5. One enzyme, one step; the enzyme binds ADP and ATP at the same time. O 1 O 2 O 3 O 4

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