If an E. coli strain contains lambda prophage, which of the following proteins you would most likely find inside this E. coli cell? CI protein а. O b. CIl protein С. N protein d. Cro protein
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Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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- Poliovirus contains a protein that possesses an NLS. A drug is developed that binds and hides this sequence on the viral protein. What happens to this viral protein, in the presence of the drug? Select all that are true. a. The viral protein is now found in the cytoplasm O b. The viral protein is found in the endomembrane system Oc. The viral protein is secreted O d. The viral protein is found in the ER O e. The viral protein can now enter the nucleusChoose the correct answer: 1. which statement is true: Select one: a. Ribosome is consists of proteins and DNA b. The E.coli ribosome has a large subunit (50S, has the 23S and a 5S RNA) and a small subunit (30S, has a 16S RNA). c.•The E.coli ribosome has a large subunit (40S, has the 23S and a 5S RNA) and a small subunit (20S, has a 16S RNA). d. The E.coli ribosome has a large subunit (60S, has the 23S and a 5S RNA) and a small subunit (40S, has a 16S RNA). 2. Which of the following are not the components of RNA? Select one: a. Adenine b. Guanine c. Thymine d. Cytosine 3. Which is the first amino group entering into urea cycle? Select one: a. Argininosuccinate b. Ornithine c. Cituilline d. carbamoyl phosphate 4. The breakdown of glycogen to form glucose occurs Select one: a. both liver and muscle b. in the brain by phosphorolysis. c. in the kidney by phosphorolysis. d. none is correct 5.The precursor to glycogen in the glycogen synthase reaction is Select one:…The CFTR membrane protein has an alpha helix that spans the membrane. The amino acid sequence of the central portion of this alpha helix, which is embedded within the membrane, is which one of the sequences below? Sequences are provided in the single letter code. а. VVVLVVVVLVVLLLVL b. EEEREEEEREERRRER C. VVVLVVVVREERRRER d. VVVEVVVVEVVEEEVE
- The CFTR membrane protein has an alpha helix that spans the membrane. The amino acid sequence of the central portion of this alpha helix, which is embedded within the membrane, is which one of the sequences below? Sequences are provided in the single letter code. a. VVVEVVVVEVVEEEVE b. VVVLVVVVLVVLLLVL c. VVVLVVVVREERRRER d. EEEREEEEREERRRERWhich of the following statements about ribosomes is correct? * a. the number of ribosomes in a cell seldom exceeds 50 b. the active site of a ribosome contains mostly protein molecules c. structurally, ribosomes have two rRNA- protein subinuts d. no correct response What is the complementary hnRNA base sequence produced from the DNA base sequence 5' C-T-A-T-A-C 3' ? * a. 3' C-T-A-G-A-T 5' b. 3' C-G-G-A-T-A 5' c. 3' G-A-U-A-U-G 5' d. 3' G-A-T-A-T-G 5' The product of the first phase in protein synthesis is * a. DNA molecules b. RNA molecules c. both RNA and DNA molecules d. neither RNA and DNA moleculesAnalysis of a mRNA sample showed that 18% of the nitrogen-base molecules present were uracil molecules. The DNA molecule that was transcribed to form the mRNA sample would most likely contain Select one: a. 18% adenine b. 18% cytosine c. 32% thymine d. 32% adenine O O O
- The picture below is the structure of a bacterial Nucleoid Associated Protein (NAP) associated with DNA that plays a critical function in organization of positioning of nucleoid. This protein is which of the following (select one)? A. Protein H-NS B. DNA Protection During Starvation (Dps) C. Histone H3 D. FIS E. Integration Host FactorA DNA antisense strand contains the following nucleotide base sequence:CGA TTT GGT TGAFrom this, what is the nucleotide sequence of the mRNA strand that is transcribed? a. CGA UUU GGU UGA b. GCU AAA CCA ACU c. GCA AAA CCA ACT d. CGA TTT CCA ACTA small section of bacterial DNA antisense strand has the following nucleotide sequence:AAT CGG TCC TCGThe tRNA anticodons for the gene sequence shown above is a. TTA GCC AGG AGC b. AAU CGG ACC ACG c. AAU CGG UCC UCG d. UUA GCC AGG AGC
- The advantage of yeast cells over bacterial cells to express human proteins is that: a. yeast cells grow faster b. yeast cells are easier to manipulate genetically c. yeast cells are eukaryotic and modify proteins similarly to human cells d. yeast cells are easily lysed to purify the proteinsIf a sequence of mRNA is CUG AGU GCA, which of the following is the DNA segment from which it was transcribed? \ a. GAC TCA CGT b. GAG TAC GCC c. CGC ATG CGG d. GAC UCA CGUList the sequence of amino acids for the strands of DNA if the 5th nucleotide is replaced with guanine. thiamin DNA TAC AGA CGG CAA CTC TGG TA CATA CGG CALA V... Kould mRNA → protein CTC TGG DNATAC CCT ATG GGA CAT GGT TAC CTT ATG mRNA → protein → 10. List the sequence of amino acids for the strands of DNA if guanine is inserted after the 5th nucleotide. DNATAC AGA CGG CAA CTC TGG TAC AGG ACG GCA ACT CTG G mRNA → protein → DNA → TAC CCT ATG GGA CAT GGT TAL mRNA → protein → DNA mRNA → protein → GGA CAT GGT. 11. List the sequence of amino acids for the strands of DNA if the 5th nucleotide is deleted. DNA → TAC AGA CGG CAA CTC TGG TAC AAC GGA mRNA → protein → Don't do before class TAC CCT ATG GGA CAT GGT ссы тат быы аса тыы т