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I need help determining the FOV for the objective microscope, ocular lens please.

I need help determining the FOV for the objective microscope, ocular lens please.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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I need help determining the FOV for the objective microscope, ocular lens please.

Total magnification is the total number of times the specimen you are viewing is magnified. To determine the total magnification you multiply the ocular lens magnification times the objective lens magnification. On your microscopes, the ocular lens always magnifies 10X. Example: 10X (ocular) x 4X (objective) = 40X (total magnification) Magnification of Objective Lens Magnification of Ocular Lens Total Magnification FOV Scanning 4x 10x 10x 10x HX1D = HOX 10 x 10 = =100x Field of View (Estimation of Size)* (Optional for BIO*121 & 122, Required for BIO*235) Total Magnification 140x 100x 400x 1000x Low Power The field of view (FOV) is the actual "circle" you see when looking in the microscope. Although this circular field of view appears to be the same no matter which objective lens you are using, this is not the case. The circular area you are actually viewing will decrease as you increase the magnification: Field of View تی G High Power 40x 10x HOX 10 = HODx Oil Immersion 100x 10x 10x100= 1,000 x
Transcribed Image Text:Total magnification is the total number of times the specimen you are viewing is magnified. To determine the total magnification you multiply the ocular lens magnification times the objective lens magnification. On your microscopes, the ocular lens always magnifies 10X. Example: 10X (ocular) x 4X (objective) = 40X (total magnification) Magnification of Objective Lens Magnification of Ocular Lens Total Magnification FOV Scanning 4x 10x 10x 10x HX1D = HOX 10 x 10 = =100x Field of View (Estimation of Size)* (Optional for BIO*121 & 122, Required for BIO*235) Total Magnification 140x 100x 400x 1000x Low Power The field of view (FOV) is the actual "circle" you see when looking in the microscope. Although this circular field of view appears to be the same no matter which objective lens you are using, this is not the case. The circular area you are actually viewing will decrease as you increase the magnification: Field of View تی G High Power 40x 10x HOX 10 = HODx Oil Immersion 100x 10x 10x100= 1,000 x
A good analogy is to imagine yourself viewing the Earth from space as you gradually move closer and closer to Middlesex. Initially your field of view is the entire western hemisphere, but as you approach the Earth's surface your field of view will progressively shrink to encompass the eastern United States, New England, Connecticut, Middletown, etc. Although your field of view is shrinking, the image in your field of view is becoming increasingly magnified. This is really no different than looking into your microscope at increasing levels of magnification. *It is also useful to know the diameter of the field of view (FOV diameter) at a particular magnification, since you can use this information to estimate the size of the specimen you are viewing. The FOV diameter at low power for your microscope (100X) is ~1.8 mm. Using this FOV diameter, you can calculate the FOV diameter at other magnifications. This is done by multiplying by the ratio of the magnifications: If you want to know the FOV diameter at 400X, you could calculate it as follows: known FOV diameter x total mag. (known FOV) = unknown FOV diameter total mag. (unknown FOV) ROCE Once you know the FOV diameter, you can estimate the dimensions of your specimen. For example, assume you are viewing the specimen below at 400X total magnification and, based on your calculation above, you know FOV diameter to be 450 µm. It appears that -4 of your specimens would fit across the FOV end to end (i.e., length = 1/4 of FOV), and -10 side to side (i.e., width = 1/10 of FOV). Thus you would estimate the dimensions of your specimen to be: 360 μ 1.8 mm x 100X/400X = 1.8 mm x 1/5 = 0.45 mm = 450 µm 500X LENGTH= 1/4 x 360 μm = 90 μm -90 μm 500X WIDTH = 1/10 x 360 μm = 36 um 0 sp fit in your F 00000 500X -36 μm Hinder in ingon
Transcribed Image Text:A good analogy is to imagine yourself viewing the Earth from space as you gradually move closer and closer to Middlesex. Initially your field of view is the entire western hemisphere, but as you approach the Earth's surface your field of view will progressively shrink to encompass the eastern United States, New England, Connecticut, Middletown, etc. Although your field of view is shrinking, the image in your field of view is becoming increasingly magnified. This is really no different than looking into your microscope at increasing levels of magnification. *It is also useful to know the diameter of the field of view (FOV diameter) at a particular magnification, since you can use this information to estimate the size of the specimen you are viewing. The FOV diameter at low power for your microscope (100X) is ~1.8 mm. Using this FOV diameter, you can calculate the FOV diameter at other magnifications. This is done by multiplying by the ratio of the magnifications: If you want to know the FOV diameter at 400X, you could calculate it as follows: known FOV diameter x total mag. (known FOV) = unknown FOV diameter total mag. (unknown FOV) ROCE Once you know the FOV diameter, you can estimate the dimensions of your specimen. For example, assume you are viewing the specimen below at 400X total magnification and, based on your calculation above, you know FOV diameter to be 450 µm. It appears that -4 of your specimens would fit across the FOV end to end (i.e., length = 1/4 of FOV), and -10 side to side (i.e., width = 1/10 of FOV). Thus you would estimate the dimensions of your specimen to be: 360 μ 1.8 mm x 100X/400X = 1.8 mm x 1/5 = 0.45 mm = 450 µm 500X LENGTH= 1/4 x 360 μm = 90 μm -90 μm 500X WIDTH = 1/10 x 360 μm = 36 um 0 sp fit in your F 00000 500X -36 μm Hinder in ingon
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