Human hormone gene Using recombinant DNA technology (described in Chapter 21), insert the coding sequence of a human hormone gene next to a sheep B-lactoglobulin promoter. This promoter is functional only in mammary cells so that the protein product is secreted into the milk. B-lactoglobulin promoter Plasmid vector Inject this DNA into a sheep oocyte. The plasmid DNA will integrate into the chromosomal DNA, resulting in the addition of the human hormone gene into the sheep's genome. Oocyte Plasmid DNA travels into the nucleus and integrates into a chromosome. Implant the fertilized oocyte into a female sheep, which then gives birth to a transgenic sheep offspring. Transgenic sheep Obtain milk from female transgenic sheep. The milk contains a human hormone. Purify the hormone from the milk.

Q: Why is the RNA polymerase/RNA/DNA duplex of the RNA polymerizing complex called transcription…

A: Transcription is the process of formation of an mRNA molecule from the DNA in the nucleus. It…

Q: Under what conditions is lac repressor bound to the lac operon?

A: Lactose operon (Lac operon) was discovered in Escherichia coli. This is studied to understand the…

Q: Explain why large amounts of transcript result when a bacterium with a lac Operon is in a medium…

A: Transcription is the process by which RNA is synthesized from DNA. The messenger RNA or mRNA…

Q: The highest level of transcription of the E. coli lac operon occurs when CAP (+cAMP) is bound to the…

A: The lac operon in E. coli is a system of genes that regulates the metabolism of lactose, a sugar.…

Q: What is the receptor for the targeting sequence?

A: Proteins synthesized by ribosomes include soluble and membrane proteins of the Endoplasmic Reticulum…

Q: A lac operon containing one mutation was cloned into a plasmid, which was introduced by…

A: (a) The plasma i gene forms such a type of repressor that will not be able to bind the inducer.

Q: What happens when a nonsense mutation is introduced into a transposon's gene encoding transposase?

A: A non-sense mutation is a mutation that terminates translation earlier resulting in the formation of…

Q: Identify the labeled factors in the figure below (A and B) and indicate the direction of…

A: Hi dear, here's your answer.can you please give me an upvote. TBP and TFIIB bind and form stable…

Q: Explain how an α helix in a transcription factor protein is able to function as a recognition helix.

A: Transcription is the first step in central dogma of protein synthesis. It involves formation of…

Q: This diagram shows a double-stranded section of DNA. The arrow indicates location and strand of the…

A: Transcription is the process of formation of mRNA using DNA as template. This is possible with the…

Q: In bacteria, genes that are often used together are controlled by a single promoter. Explain why…

A: Gene regulation refers to the numerous processes that regulate which genes are expressed and the…

Q: Why is an NFR needed at the core promoter for transcription to occur?

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: For each of the following conditions in the Lac Operon, state whether transcription will occur or…

A: The lac operon of E. coli contains genes responsible  in lactose metabolism. It is  expressed only…

Q: What is "wobble" controlled during transcription in a prokaryotic cell? Explain briefly.

A: Wobble hypothesis:  Only the first two bases of the codon have a precise pairing with the bases of…