Human hormone gene Using recombinant DNA technology (described in Chapter 21), insert the coding sequence of a human hormone gene next to a sheep B-lactoglobulin promoter. This promoter is functional only in mammary cells so that the protein product is secreted into the milk. B-lactoglobulin promoter Plasmid vector Inject this DNA into a sheep oocyte. The plasmid DNA will integrate into the chromosomal DNA, resulting in the addition of the human hormone gene into the sheep's genome. Oocyte Plasmid DNA travels into the nucleus and integrates into a chromosome. Implant the fertilized oocyte into a female sheep, which then gives birth to a transgenic sheep offspring. Transgenic sheep Obtain milk from female transgenic sheep. The milk contains a human hormone. Purify the hormone from the milk.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Why is a β-lactoglobulin promoter used?

Human
hormone
gene
Using recombinant DNA technology (described
in Chapter 21), insert the coding sequence of a
human hormone gene next to a sheep
B-lactoglobulin promoter. This promoter is
functional only in mammary cells so that the
protein product is secreted into the milk.
B-lactoglobulin
promoter
Plasmid
vector
Inject this DNA into a sheep oocyte. The
plasmid DNA will integrate into the
chromosomal DNA, resulting in the addition
of the human hormone gene into the sheep's
genome.
Oocyte
Plasmid DNA
travels into the
nucleus and
integrates into
a chromosome.
Transcribed Image Text:Human hormone gene Using recombinant DNA technology (described in Chapter 21), insert the coding sequence of a human hormone gene next to a sheep B-lactoglobulin promoter. This promoter is functional only in mammary cells so that the protein product is secreted into the milk. B-lactoglobulin promoter Plasmid vector Inject this DNA into a sheep oocyte. The plasmid DNA will integrate into the chromosomal DNA, resulting in the addition of the human hormone gene into the sheep's genome. Oocyte Plasmid DNA travels into the nucleus and integrates into a chromosome.
Implant the fertilized oocyte into a female
sheep, which then gives birth to a transgenic
sheep offspring.
Transgenic
sheep
Obtain milk from female transgenic sheep.
The milk contains a human hormone.
Purify the hormone from the milk.
Transcribed Image Text:Implant the fertilized oocyte into a female sheep, which then gives birth to a transgenic sheep offspring. Transgenic sheep Obtain milk from female transgenic sheep. The milk contains a human hormone. Purify the hormone from the milk.
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