How to determine the sequence of amino acids in a peptide chain ?
How to determine the sequence of amino acids in a peptide chain ?
Amino acids can be defined as the smallest unit which formed the proteins. They are organic molecules necessary for the synthesis of proteins. There is 22 amino acid naturally present, out of which, amino acid 21 and 22 are newly discovered.
Protein sequencing is the process that helps in determining the sequence of the amino acids in a peptide chain. The two methods of conducting protein sequencing are Edman degradation using protein sequenator and mass spectrometry.
In mass spectrometry, chemical or enzymatic degradation of the protein is done into peptides. These peptides are fractioned into high-performance liquid chromatography.
A protein sequenator is a machine that helps in identifying the sequence of amino acids in a peptide.
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