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How might concentration of antigen and/or antibody affect the formation of the blue color formation expected. in an ELISA ASSAY?
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- What is the effect of not adding the primary antibody to the ELISA reaction?What is the difference between an indirect ELISA and a sandwich ELISA? Is rapid antibody testing a form of ELISA?If you were using the ELISA to look for the presence of antibodies and the sample gave a negative result, does this mean that there were no antibodies present? Explain.
- Is rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.In ELISA, a substrate is utilized that is cleaved by the enzymatic tag on the antibody. What is this substrate and what color appears when it is cleaved?What are the different solid phases that antibody or antigen can bind to, in ELISA?
- What is the effect of not adding the antigen to the ELISA reaction?What are the two fluorescent compounds that can be used in antibody labelling?ELISA tests usually use a primary and secondary antibody. Why? What are the necessary controls one would need to perform to ensure that the antibody specificities are valid (i.e., no false-positive or falsenegative reactions)?
- To diagnosis, we used a procedure known as a "sandwich ELISA" (Enzyme-Linked Immunosorbent Assay). 1.In the "ELISA sandwich," what is the "meat"? 2.Both patients A and B had positive results. Why are these two patients' outcomes so different? 3.What is one potential cause of an ELISA false negative result? What is one potential cause of an ELISA false positive result? What is another example of how an ELISA assay should be used?Why do you coat the elisa plate with albumin?What is the label used in ELISA? Can we use different label? Why, or why not.