How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and reverse primers that bind to the same exon. B. Run a control lane where only RT was performed and not PCR. C. Run a control lane where mRNA has been amplified using PCR. D. Use forward and reverse primers that span the junction of 2 separate exons.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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How can you design your RT-PCR experiment to control for gDNA contamination?
A. Use forward and reverse primers that bind to the same exon.
B. Run a control lane where only RT was performed and not PCR.
C. Run a control lane where MRNA has been amplified using PCR.
D. Use forward and reverse primers that span the junction of 2 separate exons.
Transcribed Image Text:How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and reverse primers that bind to the same exon. B. Run a control lane where only RT was performed and not PCR. C. Run a control lane where MRNA has been amplified using PCR. D. Use forward and reverse primers that span the junction of 2 separate exons.
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