Gram positive case   on the first day back to class at the local university, a student noticed a small abscess on his right elbow but didn't think much about it. Over the next couple days, however the abscess became more swollen and painful. The student decided to visit the health centet he told the doctor it looked like a spider bite but didn't recall being bitten. The doctor took a swab of the lesion and prescribed an antibiotic cream as it looked infected. The swab was sent to the lab for testing within a few days of treatment, the student lesion was healed.       What is the overview of the disease? How did the patient most likely acquire it? How else does this organism spread to cause this disease?

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Gram positive case

 

on the first day back to class at the local university, a student noticed a small abscess on his right elbow but didn't think much about it. Over the next couple days, however the abscess became more swollen and painful. The student decided to visit the health centet he told the doctor it looked like a spider bite but didn't recall being bitten. The doctor took a swab of the lesion and prescribed an antibiotic cream as it looked infected. The swab was sent to the lab for testing within a few days of treatment, the student lesion was healed.

 

 

 

What is the overview of the disease? How did the patient most likely acquire it? How else does this organism spread to cause this disease? 

## Bacterial Identification Tests

### Bacitracin Test
An image of a petri dish containing a growth medium with streptococcus bacteria is shown. A small white disc containing bacitracin is placed in the center. The zone of inhibition (clear area around the disc) indicates sensitivity to bacitracin, which helps in the identification of bacterial species.

### Optochin Test
This image shows another petri dish, where a white disc containing optochin is placed on a bacterial lawn. The presence of a clear zone around the disc indicates sensitivity, useful for differentiating Streptococcus pneumoniae (sensitive) from other alpha-hemolytic streptococci (resistant).

### Novobiocin Test
Displayed is a petri dish with a bacterial culture. A white disc containing novobiocin is placed on the culture. The zone of inhibition around the disc helps in the identification of Staphylococcus saprophyticus (resistant) from other coagulase-negative staphylococci (sensitive).

### Catalase Test
A close-up image shows bubbles forming on a slide where bacterial cells are mixed with hydrogen peroxide. The production of bubbles indicates a positive catalase test, which differentiates Staphylococci (catalase-positive) from Streptococci (catalase-negative).

### Coagulase Test
The image shows two test tubes, one with clotted plasma (positive coagulase test) and the other with liquid plasma (negative coagulase test). This test distinguishes Staphylococcus aureus (coagulase-positive) from other Staphylococcus species (coagulase-negative).

### SBA (Sheep Blood Agar)
This image illustrates the appearance of bacterial colonies on Sheep Blood Agar (SBA). The type of hemolysis (clear zone for beta-hemolysis, greenish for alpha-hemolysis, and no change for gamma-hemolysis) around the colonies aids in bacterial identification.

### Gram Stain
An image of Gram-stained bacteria is shown under the microscope. The color and shape (purple indicating Gram-positive bacteria and pink indicating Gram-negative bacteria) help in preliminary bacterial classification. The bacteria in the image are purple, suggesting they are Gram-positive cocci.

These tests are crucial in microbiology labs for the accurate identification of bacterial species.
Transcribed Image Text:## Bacterial Identification Tests ### Bacitracin Test An image of a petri dish containing a growth medium with streptococcus bacteria is shown. A small white disc containing bacitracin is placed in the center. The zone of inhibition (clear area around the disc) indicates sensitivity to bacitracin, which helps in the identification of bacterial species. ### Optochin Test This image shows another petri dish, where a white disc containing optochin is placed on a bacterial lawn. The presence of a clear zone around the disc indicates sensitivity, useful for differentiating Streptococcus pneumoniae (sensitive) from other alpha-hemolytic streptococci (resistant). ### Novobiocin Test Displayed is a petri dish with a bacterial culture. A white disc containing novobiocin is placed on the culture. The zone of inhibition around the disc helps in the identification of Staphylococcus saprophyticus (resistant) from other coagulase-negative staphylococci (sensitive). ### Catalase Test A close-up image shows bubbles forming on a slide where bacterial cells are mixed with hydrogen peroxide. The production of bubbles indicates a positive catalase test, which differentiates Staphylococci (catalase-positive) from Streptococci (catalase-negative). ### Coagulase Test The image shows two test tubes, one with clotted plasma (positive coagulase test) and the other with liquid plasma (negative coagulase test). This test distinguishes Staphylococcus aureus (coagulase-positive) from other Staphylococcus species (coagulase-negative). ### SBA (Sheep Blood Agar) This image illustrates the appearance of bacterial colonies on Sheep Blood Agar (SBA). The type of hemolysis (clear zone for beta-hemolysis, greenish for alpha-hemolysis, and no change for gamma-hemolysis) around the colonies aids in bacterial identification. ### Gram Stain An image of Gram-stained bacteria is shown under the microscope. The color and shape (purple indicating Gram-positive bacteria and pink indicating Gram-negative bacteria) help in preliminary bacterial classification. The bacteria in the image are purple, suggesting they are Gram-positive cocci. These tests are crucial in microbiology labs for the accurate identification of bacterial species.
### Bacterial Identification Tests Chart

The table below summarizes the results of various bacterial tests used to identify different bacterial cultures. Each row presents a different type of bacteria and their results for the listed tests. The columns represent different bacteriological tests: Coagulase, Catalase, Optochin Sensitivity, Bacitracin Sensitivity, and Oxidase. The results are indicated as POS (positive), NEG (negative), or N/A (not applicable).

| Culture                         | Coagulase +/- | Catalase +/- | Optochin Sensitivity Test | Bacitracin Sensitivity +/- | Oxidase Test +/- |
|---------------------------------|---------------|--------------|---------------------------|----------------------------|------------------|
| **Staphylococcus aureus**       | POS           | POS          | N/A                       | N/A                        | N/A              |
| **Staphylococcus epidermidis**  | NEG           | NEG          | N/A                       | N/A                        | N/A              |
| **Streptococcus pyogenes**      | N/A           | NEG          | N/A                       | Zone of inhib. (+)         | N/A              |
| **Streptococcus faecalis**      | N/A           | NEG          | N/A                       | N/A                        | N/A              |
| **Streptococcus pneumoniae**    | N/A           | NEG          | Zone of inhib. (+)        | N/A                        | N/A              |
| **Micrococcus luteus**          | NEG           | POS          | N/A                       | N/A                        | N/A              |

### Test Descriptions:

1. **Coagulase Test (Coagulase +/-)**
    - **Positive (POS)**: Indicates the presence of the enzyme coagulase.
    - **Negative (NEG)**: Indicates the absence of the enzyme coagulase.
    - **N/A**: Not applicable or not tested for this bacterium.

2. **Catalase Test (Catalase +/-)**
    - **Positive (POS)**: Indicates the presence of the enzyme catalase that breaks down hydrogen peroxide.
    - **Negative (NEG)**: Indicates the absence of the enzyme catalase.
    - **N/A**: Not applicable or not tested for this bacterium.

3. **Optochin Sensitivity Test**
    - **Zone of inhib. (+)**: Indicates that the bacterium is sensitive to optochin, shown by
Transcribed Image Text:### Bacterial Identification Tests Chart The table below summarizes the results of various bacterial tests used to identify different bacterial cultures. Each row presents a different type of bacteria and their results for the listed tests. The columns represent different bacteriological tests: Coagulase, Catalase, Optochin Sensitivity, Bacitracin Sensitivity, and Oxidase. The results are indicated as POS (positive), NEG (negative), or N/A (not applicable). | Culture | Coagulase +/- | Catalase +/- | Optochin Sensitivity Test | Bacitracin Sensitivity +/- | Oxidase Test +/- | |---------------------------------|---------------|--------------|---------------------------|----------------------------|------------------| | **Staphylococcus aureus** | POS | POS | N/A | N/A | N/A | | **Staphylococcus epidermidis** | NEG | NEG | N/A | N/A | N/A | | **Streptococcus pyogenes** | N/A | NEG | N/A | Zone of inhib. (+) | N/A | | **Streptococcus faecalis** | N/A | NEG | N/A | N/A | N/A | | **Streptococcus pneumoniae** | N/A | NEG | Zone of inhib. (+) | N/A | N/A | | **Micrococcus luteus** | NEG | POS | N/A | N/A | N/A | ### Test Descriptions: 1. **Coagulase Test (Coagulase +/-)** - **Positive (POS)**: Indicates the presence of the enzyme coagulase. - **Negative (NEG)**: Indicates the absence of the enzyme coagulase. - **N/A**: Not applicable or not tested for this bacterium. 2. **Catalase Test (Catalase +/-)** - **Positive (POS)**: Indicates the presence of the enzyme catalase that breaks down hydrogen peroxide. - **Negative (NEG)**: Indicates the absence of the enzyme catalase. - **N/A**: Not applicable or not tested for this bacterium. 3. **Optochin Sensitivity Test** - **Zone of inhib. (+)**: Indicates that the bacterium is sensitive to optochin, shown by
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