Gram positive case   on the first day back to class at the local university, a student noticed a small abscess on his right elbow but didn't think much about it. Over the next couple days, however the abscess became more swollen and painful. The student decided to visit the health centet he told the doctor it looked like a spider bite but didn't recall being bitten. The doctor took a swab of the lesion and prescribed an antibiotic cream as it looked infected. The swab was sent to the lab for testing within a few days of treatment, the student lesion was healed.   what is the overview of the etiological agent?( including basic characteristics of the organism, diseases it cause and use in industry) Broad to get a sense of what this organism is

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Gram positive case

 

on the first day back to class at the local university, a student noticed a small abscess on his right elbow but didn't think much about it. Over the next couple days, however the abscess became more swollen and painful. The student decided to visit the health centet he told the doctor it looked like a spider bite but didn't recall being bitten. The doctor took a swab of the lesion and prescribed an antibiotic cream as it looked infected. The swab was sent to the lab for testing within a few days of treatment, the student lesion was healed.

 

what is the overview of the etiological agent?( including basic characteristics of the organism, diseases it cause and use in industry) Broad to get a sense of what this organism is

### Microbial Laboratory Testing Methods

#### Bacitracin Test
- Illustrates a petri dish with a clear zone of inhibition around a disk, indicating susceptibility to bacitracin.

#### Optochin Test
- Shows a blood agar plate with a clear zone around the disk, representing a positive result indicating susceptibility to optochin.

#### Novobiocin Test
- Depicts a bacterial culture with a distinct inhibition zone around a disk, demonstrating susceptibility to novobiocin.

#### Catalase Test
- Provides an image of bubbles forming on the surface of the test medium, indicating a positive catalase reaction.

#### Coagulase Test
- Displays two test tubes, one with coagulase-positive (clumping) result, demonstrating the presence of staphylococcal coagulase enzyme, and the other a negative result.

#### Gram Stain
- Shows a microscopic view of bacterial cells stained purple (gram-positive) under a microscope, highlighting their shape and arrangement.

#### Sheep Blood Agar (SBA)
- Presents a blood agar plate with visible colonies, illustrating bacterial growth and hemolytic activity.

These tests are essential for identifying and differentiating various bacterial species in microbiology.
Transcribed Image Text:### Microbial Laboratory Testing Methods #### Bacitracin Test - Illustrates a petri dish with a clear zone of inhibition around a disk, indicating susceptibility to bacitracin. #### Optochin Test - Shows a blood agar plate with a clear zone around the disk, representing a positive result indicating susceptibility to optochin. #### Novobiocin Test - Depicts a bacterial culture with a distinct inhibition zone around a disk, demonstrating susceptibility to novobiocin. #### Catalase Test - Provides an image of bubbles forming on the surface of the test medium, indicating a positive catalase reaction. #### Coagulase Test - Displays two test tubes, one with coagulase-positive (clumping) result, demonstrating the presence of staphylococcal coagulase enzyme, and the other a negative result. #### Gram Stain - Shows a microscopic view of bacterial cells stained purple (gram-positive) under a microscope, highlighting their shape and arrangement. #### Sheep Blood Agar (SBA) - Presents a blood agar plate with visible colonies, illustrating bacterial growth and hemolytic activity. These tests are essential for identifying and differentiating various bacterial species in microbiology.
### Microbial Identification Tests

The table below illustrates the results of various biochemical tests used to identify different bacterial cultures. These tests are essential for determining the specific characteristics of bacterial species and aid in diagnosing bacterial infections.

| Culture | Coagulase +/- | Catalase +/- | Optochin Sensitivity Test | Bacitracin Sensitivity +/- | Oxidase Test +/- |
|---------|---------------|--------------|---------------------------|---------------------------|-----------------|
| **Staphylococcus aureus** | POS | POS | N/A | N/A | N/A |
| **Staphylococcus epidermidis** | NEG | NEG | N/A | N/A | N/A |
| **Streptococcus pyogenes** | N/A | NEG | N/A | Zone of inhib. (+) | N/A |
| **Streptococcus faecalis** | N/A | NEG | N/A | N/A | N/A |
| **Streptococcus pneumoniae** | N/A | NEG | Zone of inhib. (+) | N/A | N/A |
| **Micrococcus luteus** | NEG | POS | N/A | N/A | N/A |

**Explanation of Columns:**

- **Coagulase +/-:** 
  - Indicates if the bacterium produces the enzyme coagulase, which causes blood plasma to clot. 
  - POS (Positive) means coagulase is present.
  - NEG (Negative) means coagulase is absent.

- **Catalase +/-:** 
  - Indicates if the bacterium produces the enzyme catalase, which breaks down hydrogen peroxide into water and oxygen.
  - POS (Positive) means catalase is present.
  - NEG (Negative) means catalase is absent.

- **Optochin Sensitivity Test:** 
  - Determines the sensitivity of bacteria to optochin, used primarily to identify Streptococcus pneumoniae.
  - Zone of inhib. (+) indicates a zone of inhibition, meaning the bacteria are sensitive to optochin.
  - N/A means not applicable or not tested.

- **Bacitracin Sensitivity +/-:** 
  - Tests the sensitivity of bacteria to bacitracin, typically used to identify Streptococcus pyogenes.
  - Zone of inhib. (+) means the bacteria are sensitive to bacitracin.
  - N/A means not applicable or not tested.

- **Oxidase Test +/-:** 
  - Identifies if the bacter
Transcribed Image Text:### Microbial Identification Tests The table below illustrates the results of various biochemical tests used to identify different bacterial cultures. These tests are essential for determining the specific characteristics of bacterial species and aid in diagnosing bacterial infections. | Culture | Coagulase +/- | Catalase +/- | Optochin Sensitivity Test | Bacitracin Sensitivity +/- | Oxidase Test +/- | |---------|---------------|--------------|---------------------------|---------------------------|-----------------| | **Staphylococcus aureus** | POS | POS | N/A | N/A | N/A | | **Staphylococcus epidermidis** | NEG | NEG | N/A | N/A | N/A | | **Streptococcus pyogenes** | N/A | NEG | N/A | Zone of inhib. (+) | N/A | | **Streptococcus faecalis** | N/A | NEG | N/A | N/A | N/A | | **Streptococcus pneumoniae** | N/A | NEG | Zone of inhib. (+) | N/A | N/A | | **Micrococcus luteus** | NEG | POS | N/A | N/A | N/A | **Explanation of Columns:** - **Coagulase +/-:** - Indicates if the bacterium produces the enzyme coagulase, which causes blood plasma to clot. - POS (Positive) means coagulase is present. - NEG (Negative) means coagulase is absent. - **Catalase +/-:** - Indicates if the bacterium produces the enzyme catalase, which breaks down hydrogen peroxide into water and oxygen. - POS (Positive) means catalase is present. - NEG (Negative) means catalase is absent. - **Optochin Sensitivity Test:** - Determines the sensitivity of bacteria to optochin, used primarily to identify Streptococcus pneumoniae. - Zone of inhib. (+) indicates a zone of inhibition, meaning the bacteria are sensitive to optochin. - N/A means not applicable or not tested. - **Bacitracin Sensitivity +/-:** - Tests the sensitivity of bacteria to bacitracin, typically used to identify Streptococcus pyogenes. - Zone of inhib. (+) means the bacteria are sensitive to bacitracin. - N/A means not applicable or not tested. - **Oxidase Test +/-:** - Identifies if the bacter
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