Given a solution of 0.200 M HEPES in its fully protonated form, and ready access to 0.150 MHCI, 0.150 M NaOH and distilled water, complete the questions below to describe the preparation of 1 L of 0.0400 M HEPES buffer solution at pH 7.90. Volume of stock HEPES needed = 0.200 L or 200 mL i) Calculate the total volume of 0.150 M NaOH that needs to be added to make the buffer %3| system. mL ii) Calculate the volume of distilled water that needs to be added to make the solution up to 1 L.

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Chapter1: Chemical Foundations
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Given a solution of 0.200 M HEPES in its fully
protonated form, and ready access to 0.150
M HCI, 0.150 M NAOH and distilled water,
complete the questions below to describe the
preparation of 1 L of 0.0400 M HEPES buffer
solution at pH 7.90. Volume of stock HEPES
needed = 0.200 L or 200 mL
i) Calculate the total volume of 0.150 M NaOH
that needs to be added to make the buffer
%3D
system.
mL
ii) Calculate the volume of distilled water that
needs to be added to make the solution up to
1 L.
mL
Transcribed Image Text:Given a solution of 0.200 M HEPES in its fully protonated form, and ready access to 0.150 M HCI, 0.150 M NAOH and distilled water, complete the questions below to describe the preparation of 1 L of 0.0400 M HEPES buffer solution at pH 7.90. Volume of stock HEPES needed = 0.200 L or 200 mL i) Calculate the total volume of 0.150 M NaOH that needs to be added to make the buffer %3D system. mL ii) Calculate the volume of distilled water that needs to be added to make the solution up to 1 L. mL
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