Give specific procedure ( tell the measurement needed ) based on this using yeast , sugar and all home equipments only (container, oven )

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Give specific procedure ( tell the measurement needed ) based on this using yeast , sugar and all home equipments only (container, oven )
Aseptic technique is a set of routine measures that are taken to prevent
cultures from being contaminated by unwanted microorganisms. Examples
of aseptic techniques are:
-Sterilized all containers used for inoculation to avoid any possible
i.
contamination.
-Cleaning and disinfecting work surfaces prior to use
-Limiting the duration that cultures or media are uncapped and exposed to
the air
-Keeping containers closed whenever possible
-Effectively sterilizing inoculating loops/tools that comes into contact with
cultures or media, and
-Avoiding breathing on cultures or sterile instruments/tools.
Preparation of medium with carbon source
Preparation of additional nutrient
Preparation of inoculum (can be either yeast or natural bacterial from natural
sources for instance dry yeast or fruit bacteria or any other kind)
Inoculation technique
Sampling growth at initial time: t = 0 hour
Sampling growth at you selected interval of time for instance every 5 hours
or etc. Please choose accordingly so as to ensure at least 5 minimum data
is collected considering lag phase, growth phase, stationary phase, and
death phase.
i.
iii.
iv.
V.
vi.
vii.
viii.
Qualitative observation
Quantitative observation for this case cell dry weight (CDW)
ix
Transcribed Image Text:Aseptic technique is a set of routine measures that are taken to prevent cultures from being contaminated by unwanted microorganisms. Examples of aseptic techniques are: -Sterilized all containers used for inoculation to avoid any possible i. contamination. -Cleaning and disinfecting work surfaces prior to use -Limiting the duration that cultures or media are uncapped and exposed to the air -Keeping containers closed whenever possible -Effectively sterilizing inoculating loops/tools that comes into contact with cultures or media, and -Avoiding breathing on cultures or sterile instruments/tools. Preparation of medium with carbon source Preparation of additional nutrient Preparation of inoculum (can be either yeast or natural bacterial from natural sources for instance dry yeast or fruit bacteria or any other kind) Inoculation technique Sampling growth at initial time: t = 0 hour Sampling growth at you selected interval of time for instance every 5 hours or etc. Please choose accordingly so as to ensure at least 5 minimum data is collected considering lag phase, growth phase, stationary phase, and death phase. i. iii. iv. V. vi. vii. viii. Qualitative observation Quantitative observation for this case cell dry weight (CDW) ix
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