From the study with UV-Vis spectroscopy, four samples of solutions with different known concentrations of atrazine were analyzed, using a 1 cm optical path cuvette at 220 nm. The analytical curve (calibration curve) with absorbance values (y-axis) per atrazine concentration (x-axis) provided a straight line with the following parameters: linear coefficient = 0 and slope = 760. assumptions of the Lambert-Beer Law, evaluate the following statements: I. The molar absorptivity coefficient (E), under the described experimental conditions, cannot be determined. II. A sample of a solution containing atrazine with an absorbance of 0.2 will show a concentration of 2.6x10-4 mol/L. III. Atrazine concentrations could be determined due to absorption taking place in the visible region. IV. The change in absorbance as a function of the analyte concentration is directly related to the absorptivity coefficient of atrazine and the length of the optical path of the cuvette. It is correct only what is stated in: I e III I, II e IV III e IV I, II e III II e IV
From the study with UV-Vis spectroscopy, four samples of solutions with different known concentrations of atrazine were analyzed, using a 1 cm optical path cuvette at 220 nm. The analytical curve (calibration curve) with absorbance values (y-axis) per atrazine concentration (x-axis) provided a straight line with the following parameters: linear coefficient = 0 and slope = 760. assumptions of the Lambert-Beer Law, evaluate the following statements:
I. The molar absorptivity coefficient (E), under the described experimental conditions, cannot be determined.
II. A sample of a solution containing atrazine with an absorbance of 0.2 will show a concentration of 2.6x10-4 mol/L.
III. Atrazine concentrations could be determined due to absorption taking place in the visible region.
IV. The change in absorbance as a function of the analyte concentration is directly related to the absorptivity coefficient of atrazine and the length of the optical path of the cuvette. It is correct only what is stated in:
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