Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
1. The standard curve was prepared using BSA as a standard with a concentration of (2.0 mg/ml, 1ml). Fill in the table below and show the calculations for determining the final BSA concentration in each vial.
Formula C1V1=C2V2
Transcribed Image Text:Table 2. Preparation of Diluted Bovine Serum Albumin (BSA) Standards for Standard Test Tube
Protocol.
A
B
C
D
E
F
G
H
I
Vial
Volume of Diluent:
RIPA buffer (µL)
0
125
325
175
325
325
325
400
400
Volume and Source
of BSA (μL)
300 of Stock
375 of Stock
325 of Stock
175 of vial B
dilution
325 of vial C
dilution
325 of vial E dilution
325 of vial dilution
100 of vial G
dilution
0
Final BSA
Concentration
(µg/mL)
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