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1. The standard curve was prepared using BSA as a standard with a concentration of (2.0 mg/ml, 1ml). Fill in the table below and show the calculations for determining the final BSA concentration in each vial.
Formula C1V1=C2V2
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- Draw the steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 mL of 1X diluent or saline in a test tube. Pipet 50 µl of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.Draw these steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 ml of 1X diluent or saline in a test tube. Pipet 50 µul of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.Compute for the required amount of the ingredients necessary to make 20g suppository on 105g (not 100g as shown on the table below.) Ingredients Original Amount Computed Amount Glycerin (1.249 g/mL) 91.0 g Sodium Stearate 9.00 g Water (1 g/mL) 5.0 g To make 100 grams Additionally, the photo shows the computation to make 30 g suppositories in 100 g.
- The physician orders Mesna 0.2 g IV for the patient. Select the dosage strength that requires no conversion in solving for the ordered dose. NDC 25021-201-10 Rronly MESNA INJECTION 10 mL 1g g per (100 mg per mL) For IV Use 10 mL Multi-Dose Vial SAGENT 100 mg 1 mL 1 g O 10 mL 100 mg mL 1 g 1 mL LATEX-FREE Sterile, Nonpyrogenic. Each mL contains: 100 mg of mesna, 0.25 mg of edetate disodium, sodium hydroxide to adjust the pH to (6.5 to 7.4) and q.s. with Water for Injection. 10.4 mg of benzyl alcohol added as a preservative. Usual Dosage: See insert for dosing information. Store at 20° to 25°C (68° to 77°F). [See USP Controlled Room Temperature].The figures 1 and 2 show the agarose- gel electrophoresis pattern of the serum proteins of a healthy cow and sheep. Discuss briefly on the components of the serum proteins from these two animals. Albumin a az Fig. 1. Representative agar gel electrophoretic pattern of serum sample from a healthy cow Albumin | Fig. 2. Representative agar gel electrophoretic pattem of serum sample from a healthy sheepHello, i'm having a hard time organzing the calculations when it comes to rate mL/h. I already solved this problem but I need your help to confirm if my calculations were correct. I would appreciate if you can add step by steps in order for me to understand it better, thank you! Order: Pulmocare 480 mg daily via PEG over 6 hours, followed by 50 mL sterile water flush. 1. The Pulmocare label states 100 mg/100 mL. At what rate in mL/h will you set the feeding pump to administer the Pulmocare? 2. Calculate the total amount of enteral fluid the patient will receive in 24h 3. How many milliliters of IV fluids is the patient receiving in 24h?
- The antibiotic Keflex is available in a solution with a concentration of 250 mg per 5 mL of solution or in a250 mg capsules. The recommended dosage is 25 mg/kg/day. a. How many capsules should a 40-kg person take every six hours? b. Suppos the antibiotic solution is given intravenously to a 40-kg person over a six-hour period, using a microdrop system with a drop factor of 60 ggt/mL (60 drops/mL). What is the drop rate that should be used in units of ggt/hr (drops/hr)?900ml of lactated ringers is infusing at a rate of 42gtt/min. The drop factor is 60gtt/ml. How long will it take for the IV to infuse in hours/minutes? using dimensional anaylisis and show workWhat is the essence of the control band is pregnancy (HCG) test?
- Draw the steps of Qualitative Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR 1. Bring the test reagents and samples to room temperature (Note 1). 2. Shake the latex vial gently. Aspirate dropper several times to obtain a thorough mixing. 3. Place 1 drop (50 µL) of the serum under test into one of the circles on the card. Dispense 1 drop of positive control serum and 1 drop of negative control into two additional circles. 4. Add 1 drop of ASLO-Latex Antigen to each circle next to the sample to be tested. 5. Mix the contents of each circle with a disposable stirrer while spreading over the entire area enclosed by the ring. Use separate stirrers for each mixture. 6. Rotate the slide by means of a mechanical rotator (100 r.p.m.) for a period of 2 minutes (Note 2). 7. Observe immediately under a suitable light source for any degree of agglutination.2.4. Preparation of a Calibration CurveStandard Bovine Serum Albumin (BSA) solutionStock solution: 2.00 mg/mLStandard solutions: 1.00 mL of 0, 0.125, 0.25, 0.5, 1.0, 1.5 and 2.0 mg/mL BSADiluent: distilled water a. on the picture b.Using a micropipette, obtain 62.5 μL of 2.00 mg/mL stock BSA solution, then add 937.5 μL ofdistilled water. Mix using a vortex mixer.c. Complete Table 2.4 on your own. Calculate the volumes needed for preparing 1.00 mL of theremaining concentrations following the sample calculation. Table 2.4. Volumes needed for the preparation of the BSA standard solutions. Concentration to beprepared, mg/mL Volume of 2.00 mg/mLstock solution needed, μL Volume of distilledwater needed, μL 0.00 0 1000.00 0.125 62.5 937.5 0.25 0.50 1.00 1.50 2.00 please complete the given table with the correct computation like in the pictureOrder: Diltiazem HCI 125 mg in 100 mL D5W to infuse at 20 mg/hr. Available: only Usual Dosage: See package insert. DISCARD UNUSED PORTION. Non-latex. Store under refrigeration 2-8°C (36-46°F). Date removed from refrigeration NDC 10019-510-79 Diltiazem HCI Injection Date to be discarded 25 mg/5 mL (5 mg/mL) Baxter Healthcare Corporation FOR DIRECTIV BOLUS INJECTION Deerfield, IL 60015 USA 462-463-01 Determine the following: a. How many milliliters will you add to the IV? (Enter numeric value only.) mL (01)00310019510798 b. Determine the rate in mL/hr. (Consider the medication in the volume.) mL/hr
