For the binding graph of Protein B with ligand X on the right, (i) Estimate the K, for Protein B with ligand X. Explain how you determined the K. (ii) Protein B binds to ligand Z with a Kg = 4.8 x 10+. Display a curve on the graph to represent Protein B binding to ligand Z. Using the information you have, explain which ligand Protein B has higher affinity for.
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
![For the binding graph of Protein B with ligand X on the
right, (i) Estimate the K, for Protein B with ligand X.
Explain how you determined the Ka. (ii) Protein B
binds to ligand Z with a Ka = 4.8 x 106. Display a curve
on the graph to represent Protein B binding to ligand Z.
Using the information you have, explain which ligand
Protein B has higher affinity for.
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[Ligand] umol L
An uncatalyzed reaction has AG = 59.5 kJ mol and a rate of reaction that is 6.31 x 109 umol s1 at 25.0 °C
and pH 8.1. When an enzyme is added, the rate of reaction is 1.49 umol s' at 25.0 °C and pH 8.1.
Calculate the activation energy of the catalyzed reaction.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F14c432fb-49ed-49ed-a7d2-bd3c5f79fdeb%2F98ab0181-8e3b-4955-8a3b-f09b21f211ca%2Fti73m5_processed.jpeg&w=3840&q=75)
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