Figure Q17-1 Analysis of Hoechst 33342 fluorescence in a population of cells sorted in a flow cytometer (Problem 17-8). 2 relative fluorescence per cell number of cel
Figure Q17-1 Analysis of Hoechst 33342 fluorescence in a population of cells sorted in a flow cytometer (Problem 17-8). 2 relative fluorescence per cell number of cel
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Hoechst 33342 is a membrane-permeant dye that
fluoresces when it binds to DNA. When a population of
cells is incubated briefly with Hoechst dye and then sorted
in a flow cytometer, which measures the fluorescence of
each cell, the cells display various levels of fluorescence as
shown in Figure Q17–1.

Transcribed Image Text:Figure Q17-1 Analysis of Hoechst 33342
fluorescence in a population of cells sorted
in a flow cytometer (Problem 17-8).
2
relative fluorescence per cell
number of cel
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