f you are provided with a stock solution of 0.5 M DTT, how much of that stock solution is required for this same solution (listed below)? 1.5 liters of a 1.8 M phosphate buffer (pH 7.2) containing 1.2% sucrose (w/v), 0.5 M NaCl, and 1mM DTT. Useful information: pka of secondary phosphate = 6.8 Formula weights (FW) of compounds: NaH₂PO4.H₂O FW = 138 Na2HPO4. 2H₂O FW = 178 Sucrose FW= 342.3 30 ml 20 ml 0.3 ml 3 ml 2 ml
f you are provided with a stock solution of 0.5 M DTT, how much of that stock solution is required for this same solution (listed below)? 1.5 liters of a 1.8 M phosphate buffer (pH 7.2) containing 1.2% sucrose (w/v), 0.5 M NaCl, and 1mM DTT. Useful information: pka of secondary phosphate = 6.8 Formula weights (FW) of compounds: NaH₂PO4.H₂O FW = 138 Na2HPO4. 2H₂O FW = 178 Sucrose FW= 342.3 30 ml 20 ml 0.3 ml 3 ml 2 ml
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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
Transcribed Image Text:If you are provided with a stock solution of 0.5 M DTT, how much of that stock solution
is required for this same solution (listed below)?
1.5 liters of a 1.8 M phosphate buffer (pH 7.2) containing 1.2% sucrose (w/v), 0.5 M
NaCl, and 1mM DTT.
Useful information:
pKa of secondary phosphate = 6.8
Formula weights (FW) of compounds:
NaH₂PO4.H₂O FW = 138
Na₂HPO4. 2H₂O FW = 178
Sucrose
FW=342.3
30 ml
20 ml
0.3 ml
3 ml
O2 ml
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