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- fill in the volumes required to make the various dilutions of Coomassie Blue and their respective concentrations. show sample calculations.Define the term buffer. Explain the difference between carbonic acid (H2CO3) and hydrochloric acid (HCl). Can they both be used as buffers? Why or why not.Make a list of the five most critical qualities of an effective extraction buffer. Please provide a brief description of each.
- Ammonia-containing window cleaners are considered pH-neutral substances true or false?Using the Henderson-Hasselbalch equation, calculate the pH of a buffer solution made from 0.20 M CH3COOH and 0.050 M CH3COO- that has pk3= 4.7. 4.1 0.4 None of the answers is correct 2.5 5.3What is the pH of the solution given the following information? Methyl orange = yellow Methyl red = yellow Bromothymol blue = green Note: use the provided reference table for color indicators below as a guide pH 5 pH 8 pH 7 pH 6
- what are the buffers used for biological and clinical measurements? state the properties of each buffer and the limitation to its applications.From the Henderson-Hasselbach equation, calculate the ratio of dihydrogen phosphate (H2PO4-) and monohydrogen phosphate (HPO4-2) components required to produce buffer solution with1. pH 6.22. pH 7.23. pH 8.2Draw titration curve for compound X when NaOH equivalence(s) is/are added. Indicate the buffering region(s) with a box.
- How does the results of the pH meter and the pH paper compare with each other?Normal arterial pH is 7.35 to 7. 45. Both metabolic and respiratory alkalosis have pH more basic compare to the normal arterial pH. The difference is 0.5 units. What is pH value in alkalosis? Niemann-Pick type C disease is a progressive neurological disease; symptoms include unsteady walking with uncoordinated limb movements, slurred speech, a difficulty in swallowing, epilepsy and tremor. This condition is due to the abnormal functioning of a lysosome. State the normal functions performed by this organelle.The glass electrode used in commercial pH meters gives an electrical response proportional to the concentration of hydrogen ion. To convert these responses to a pH reading, the electrode must be calibrated against standard solutions of known H+ concentration. Determine the weight in grams of sodium dihydrogen phosphate (NaH2PO4 · H2O; FW 138) and disodium hydrogen phosphate (Na2HPO4 ; FW 142) needed to prepare 1 L of a standard buffer at pH 7.00 with a total phosphate concentration of 0.100 M . See Problem 24 for the pKa values of phosphoric acid.