Explain the diauxic growth of cells with the help of growth of E.Coli in batch culture containing multi-substrates.

Q: Why is GAPDH not be a suitable loading control for nuclear extracts?

A: * In many molecular biology assays, such as Western blotting, the housekeeping gene glyceraldehyde…

Q: Derive a discussion and conclusion for E.coli growth rates at different temperatures and time for…

A: The rod-shaped, gram-negative bacterium Escherichia coli is a member of the Enterobacteriaceae…

Q: Describe the events that occur with E. coli in each of the following growth conditions: in a medium…

A: E. coli is a bacteria that has about three thousand genes. The bacteria E. coli has a set of three…

Q: Compare the lysis for bacteria to that for CHO (Chinese Hamster Ovary) cells. Note, CHO cells are…

A: Introduction :- Lysis refers to the breaking open of cells to release their contents. This can be…

Q: Discuss the benefit and drawbacks of using cell suspension culture for the production of important…

A: Secondary metabolites are phytochemicals or chemical that plants itself synthesized.Plants does not…

Q: Which medium is well-indicated to amplify a small population of bacteria mixed with much larger…

A: The goal is to find a suitable medium for amplifying a small population of bacteria together with…

Q: You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a…

A: A serial dilution is a laboratory technique that uses a series of consecutive dilution steps to…

Q: What is the mechanism of the reaction of the Bradford reagent with proteins? (explain in not less…

A: The Bradford protein assay is used to measure the concentration of total protein in a sample.

Q: the FimH crystal structure contains a nickel ion. Predict what might have happened if a…

A: Adhesion can be described as the phenomenon by which bacterial protein, as well as carbohydrates,…

Q: explain the possible factors affecting the growth of e.coli that results to no growth after doubling…

A: Bacterial growth are equated to cell number- one bacteria divides into two, then these two produce…

Q: Differentiate between the de novo synthesis of nucleotides and salvage - pathway synthesis.

A: Both the pathways include the synthesis of compounds or large biomolecules. The salvage pathway…

Q: Which statement is false? An auxotrophic mutant requires at least one nutrient for growth Bacterial…

A: Introduction  Bacteria are a type of unicellular microorganisms that belong to the domain Bacteria.…

Q: What is the purpose of the negative and positive control in the Disc diffusion assay? Why is it…

A: The disc diffusion assay is used to determine the susceptibility of test bacteria to different…

Q: Describe in detail, the key steps of the urea cycle.

A: Steps of Urea Cycle: Formation of Carbamoyl Phosphate Synthesis of Citrulline Synthesis of…

Q: Describe the four steps that are usually carried out in micropropagation

A: BASIC INFORMATION    TISSUE CULTURE   In this a tissue is  made to grow in the laboratories that in…

Q: Which molecules does E. coli not have to synthesize when grown in LB, compared to minimal media?…

A:

Q: How is the specified substrate determined?

A: An enzyme is a biological substance that acts as a catalyst in living organisms, regulating the rate…

Q: Why are proteins purified? Explain with examples

A: Protein purification is a series of processes intended to isolate a single type of protein from a…

Q: Discuss the concept of microwave fixation and cryofixation, and why it is suitable for…

A: Microwave fixation and cryofixation are the bilogical techniques which are used for stabilising th⁶e…

Q: Explain why bacterial chemotaxis is referred to as a “biased random walk”

A: Chemotaxis is the movement of an organism in response to a chemical stimulus which can be attractant…

Q: Could any one provide me with the protocol for Plasma Membrane Isolation via sucrose density…

A: Ultracentrifugation is the technique of high-speed centrifugation in which intracellular components…

Q: Describe the molecular mechanism by which quorumsensing controls bioluminescence in V. fischeri.

A: The bacterial cell communicates with each other using signaling molecule secreted by them concerning…

Q: Determine the appropriate bacterial strain that can be allowed to mate with the strain that is…

A: An organism's genotype is influenced by its genome as well as by its environment. It is not as…

Q: Define growth. Why does the lag phase in some bacteria is short or absent and the log phase of the…

A: Grоwth, the inсreаses in сell size аnd number thаt tаke рlасe during the life histоry оf аn оrgаnism…

Q: What other method could be used to purify lysozyme from white using its unusual pl?

A: The unique charge properties of lysozyme with a very high pI of about 10.5 can be used in the…

Q: For growth, a mutant bacterium requires that an amino acid not required by the wild parental strain…

A: Answer :- For growth, a mutant bacterium requires that an amino acid not required by the wild…

Q: Then there are the drugs that inhibit metabolic pathways. Describe how sulfa drugs act to prevent…

A: Sulfa drugs, also known as sulphonamide, are a type of synthetic antibiotics. It is used in the…

Q: Using ultrafiltration, you concentrate a solution of Protein X as follows: Initial volume protein X…

A: Ultrafiltration is a laboratory technique used to remove minute-level impurities from biological…

Q: a. For the E.coli haploid genotype below, determine the phenotype for the following proteins. Assume…

A: The lactose operon is an example of inducable operon in which the expression (or transcription) of…

Q: subcellular fractionatio

A: Centrifugation:  Molecules with different densities are separated by rotating them in a solution…

Q: The following questions refer to membrane protein isolation A) What is the difficulty when working…

A: Proteins are considered biological, organic polymers made of amino acids. Amino acids are connected…

Q: In bacterial transforamtion, how should P+ and P- tubes look like after a successful transformation?

A: The tranformation process refers to introduction of foreign DNA in the bacteria cells. It is a type…

Q: Hypothetically make the Dichotomous key for Micrococcus luteus, Bacillus subtilis, Bacillus,…

A: A dichotomous key is a tool that allows the user to determine the identity of organisms based on a…

Q: Using a schematic diagram, summarize the following steps in preparing competent cells for…

A: prepare competent cells for transformation ;- Add 20 µL of each ligation reaction or 1 ng of uncut…

Q: How many microliters of a 2.5X restriction enzyme buffer would be required if the total volume of…

A: Restriction enzyme buffer: Buffer selection is very important for obtaining the high activity of…

Q: What are the components of a carbon-free broth or minimum medium for E.coli ? An example for broth…

A: To find: The components of carbon free broth for E.coli. Example for broth. E .coli is a gram…

Q: What is the average time period for an E. coli culture to go from lag phase to death phase?  Is it…

A: Lag phase is the the delay before the start of exponential growth. Death phase: when cell loses…