Explain one by one why the results is like that for 4 test tubes in pancreatin digestion specifically for protein digestion B. Pancreatin digestion1. Prepare 4 test tubes as follows:Tube 1 - 3 ml neutral pancreatinTube 2 - 3 ml waterTube 3 - 3 ml water + 1.5 ml pancreatinTube 4- 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% Na2CO32. Place equal size of hard-boiled egg white in all tubes.3. Place all tubes in water bath with a temperature maintained at 40°C_for 1.5hours.4. Add 3 drops of toluene in all tubes and incubate till the next lab period.5. Observe the size of the egg white in each of the tubes.6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubeslabelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CUSO4 in eachtube. Mix and compare the result of the 4 tubes. B. Pancreatin DigestionTUBESIZE of egg whiteResultsPurple colored Solution1(Pancreatin)No change in sizeNo change in sizePurple colored Solution2(Water)No change in sizeLight-Purple colored Solution3(water, Pancreatin)(Ctrl) -Blue colored solutionSize decreased4(water, Pancreatin,Na2CO3)greatly

Pancreatin is a medication which is mixture of various digestive enzymes. These enzymes are…

Answered: Explain one by one why the results is…

Explain one by one why the results is like that for 4 test tubes in pancreatin digestion specifically for protein digestion

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Explain one by one why the results is like that for 4 test tubes in pancreatin digestion specifically for protein digestion

B. Pancreatin digestion
1. Prepare 4 test tubes as follows:
Tube 1 - 3 ml neutral pancreatin
Tube 2 - 3 ml water
Tube 3 - 3 ml water + 1.5 ml pancreatin
Tube 4
- 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% Na2CO3
2. Place equal size of hard-boiled egg white in all tubes.
3. Place all tubes in water bath with a temperature maintained at 40°C_for 1.5
hours.
4. Add 3 drops of toluene in all tubes and incubate till the next lab period.
5. Observe the size of the egg white in each of the tubes.
6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubes
labelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CUSO4 in each
tube. Mix and compare the result of the 4 tubes.

B. Pancreatin Digestion
TUBE
SIZE of egg white
Results
Purple colored Solution
1(Pancreatin)
No change in size
No change in size
Purple colored Solution
2(Water)
No change in size
Light-Purple colored Solution
3(water, Pancreatin)
(Ctrl) -
Blue colored solution
Size decreased
4(water, Pancreatin,
Na2CO3)
greatly

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