Explain each section of the flowchart in detail by integrating all the concepts. The explanation should be focused on the strategies and their rationale rather than the procedures and protocols. Explain the integration of one subtopic with others with reference to the flow chart in detail. RestrictionendonucleaseDuplexCDNADirectchemicalsynthesisDNAMechanicalPCRfragmentsshearingdigestionsynthesisBlunt-endLigationcohesiveTAJoining tovectorHomopolymertailingligation(no linker)LinkermoleculescloningterminiIntroductioninto host cellTransfectionwith recombinantTransformationwith recombinantplasmid DNAIn vitro packaging into phagecoat: transduction withrecombinant phage or cosmidphage DNAProtein-proteininteractionsProtein-ligandinteractionsFunctionalGain ofSelection orscreeningHybridizationPCRImmunochemicalcomplementationfunction

The process by which identical copies of DNA (deoxyribonucleic acid) or fragments of DNA are…

Explain each section of the flowchart in detail by integrating all the concepts. The explanation should be focused on the strategies and their rationale rather than the procedures and protocols. Explain the integration of one subtopic with others with reference to the flow chart in detail.

Explain each section of the flowchart in detail by integrating all the concepts. The explanation should be focused on the strategies and their rationale rather than the procedures and protocols. Explain the integration of one subtopic with others with reference to the flow chart in detail.

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter20: Viruses, Bacteria, And Archaea

Section: Chapter Questions

Problem 6SQ

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Question

100%

Restriction
endonuclease
Duplex
CDNA
Direct
chemical
synthesis
DNA
Mechanical
PCR
fragments
shearing
digestion
synthesis
Blunt-end
Ligation
cohesive
TA
Joining to
vector
Homopolymer
tailing
ligation
(no linker)
Linker
molecules
cloning
termini
Introduction
into host cell
Transfection
with recombinant
Transformation
with recombinant
plasmid DNA
In vitro packaging into phage
coat: transduction with
recombinant phage or cosmid
phage DNA
Protein-protein
interactions
Protein-ligand
interactions
Functional
Gain of
Selection or
screening
Hybridization
PCR
Immunochemical
complementation
function

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