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Explain A cell-cycle mutant of yeast grown at restrictive temperature?
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- Determine the phenotypes of grande and petite yeast cells with respect to the extraction of cell energy from the environment.How long can yeast remain dormant?What is the ploidy and equation for a typical S. pombe cell? Describe specifically how the ploidy of the yeast cells, and the phase in which the cells have been arrested, will increase the success of creating cells that will express the phenotype of the silenced target gene.
- Anaphase B involves the separation of spindle poles. What forces have been proposed to drive this separation? What underlying molecular mechanisms are thought to provide these forces?What is the accurate separation of duplicatedchromosomes—involves a molecular machine comprisingdynamic microtubules and microtubule-associated motors.please answer (i)
- Stable transmission of linear yeast artificial chromosomes(YACs) requires?Yeast cells are eukaryotes, and they require a functional secretory pathway to grow and to maintain cellular organization. But surprisingly, when SRP is removed from yeast cells by deleting the relevant genes, the cells are still alive (although they grow slowly). a) How can yeast survive without SRP? Propose two alternative hypotheses. b) How might electron microscopy of normal and SRP-deficient yeast cells help you to distinguish between these two hypotheses?Before JCVI-syn3.0 was produced, what steps were used to create a synthetic Mycoplasma cell? Put the events below into the correct order. Drag and drop the events into the proper sequence from left to right. ▸ View Available Hint(s) First Step Transformed cells are detected by the presence of lacz, which causes them to cleave Xgal and produce a blue color. Yeast is transformed using Mycoplasma mycoides fragments. The synthetic chromosome is transformed into Mycoplasma capricolum. The synthetic Mycoplasma mycoides chromosome is purified. DNA fragments assemble through homologous recombination in yeast. Reset Help Last Step
- please do (ii)Consider the figure below showing how the concentration of four cyclins (comp A to comp D) vary throughout the cell cycle. Comp B Comp C Comp D Comp A G1 Phase S Phase G2 Phase Mitosis i) Why does the concentration of different cyclins vary throughout the cell cycle? ií) which of the four cyclins shown represents the G1 cyclin? What is the function of this cyclin? ConcentrationWhat cellular components are involved in the steep decline in the amount of cyclin B when the wild-type cells exit from mitosis?
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