Experimenters can separate F-actin thin myofilaments frommyosin thick myofilaments. First they homogenize musclecells in a blender (to break cell membranes); then they place thehomogenate in a Ca2+-free “relaxing solution” that contains ATP.Explain why ATP must be present and Ca2+ ions must not bepresent in order to isolate thick and thin myofilaments from eachother.
Experimenters can separate F-actin thin myofilaments frommyosin thick myofilaments. First they homogenize musclecells in a blender (to break cell membranes); then they place thehomogenate in a Ca2+-free “relaxing solution” that contains ATP.Explain why ATP must be present and Ca2+ ions must not bepresent in order to isolate thick and thin myofilaments from eachother.
Human Physiology: From Cells to Systems (MindTap Course List)
9th Edition
ISBN:9781285866932
Author:Lauralee Sherwood
Publisher:Lauralee Sherwood
Chapter8: Muscle Physiology
Section: Chapter Questions
Problem 2TAHL
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Experimenters can separate F-actin thin myofilaments from
myosin thick myofilaments. First they homogenize muscle
cells in a blender (to break cell membranes); then they place the
homogenate in a Ca2+-free “relaxing solution” that contains ATP.
Explain why ATP must be present and Ca2+ ions must not be
present in order to isolate thick and thin myofilaments from each
other.
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