Experiment used to confirm the type of inhibition exerted by AZT on the HIV reverse transcriptase enzyme.
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Q: Literature on the type of inhibition exerted by AZT on the HIV reverse transcriptase enzyme.
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Q: Part A What kind of inhibition is imposed on HIV protease by ritonavir? O irreversible inhibition O…
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Q: How can one confirm/investigate the type of inhibition exerted by AZT on the HIV reverse…
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- Part A What kind of inhibition is imposed on HIV protease by ritonavir? O irreversible inhibition O reversible inhibition O uncompetitive inhibition. Submit Request AnswerAzidothymidine is a Thymidine analog used to inhibit viral reverse transcriptase. Explain the application of this nucleoside analog as an antiviral drug and how it affects reverse transcription and therefore block the ability of the virus to hijack the host cellTransduction is sometimes described as a mistake in the bacteriophage reproductive cycle. Explain how it can be viewed as a mistake.
- Detail of basic steps involved in synthesis of a recombinant drug product. Justify by manufacturing protocol of recombinant insulin.When Zidovudine (3'-azidothymidine or AZT) is added directly to an assay measuring the rate of DNA synthesis catalyzed by the AIDS virus reversed transcriptase, AZT is shown to be without significant inhibitory activity. Explain how AZT is metabolized in virus infected cells to convert the nucleoside into a potent and effective reversed transcriptase inhibitor.What does the enzyme reverse transcriptase do and why is it agood target for anti-HIV drugs?
- A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted 4 times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 21 plaques. What is the initial density of bacteriophages in the original 1 mL? Recall that initial phage density = (plaque number/mL) ×× (dilution factor).Explain the effect of COM-blockers on bacterial transformation and their application in controlling drug resistance.Describe how the one step growth experiment demonstrates the events that take place during the lytic cycle of bacteriophage
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