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Experiment #1 From Antoinette de Senna's Master's thesis on "Screening of biological control organisms for the management of phytopathogenic fungi and foodborne pathogens on produce". Objective: To screen three LAB (Lactobacillus plantarum, Pediecoccus acidilactici, and Pediococcus pentosaceus) for antimicrobial activity against the foodborne pathogens Listeria monocytogenes, Salmonella, and Escherichia coli. Methods: 1 ml of the pathogen was placed into a petri dish. Then. 15-20 mi molten tryptic soy agar (TSA) tempered to 50°C was added. When the TSA solidified, a loopful of LAB was spotted onto the agar. Plates were incubated at 35°C for 24 hours then the zone of inhibition was measured from the boarder of the bacterial colony to the perimeter of the clkaring. Results: E col OIS7H7 Questions: 1. What is the independent variable? 2. What is the dependent variable? P. pontonv

Experiment #1 From Antoinette de Senna's Master's thesis on "Screening of biological control organisms for the management of phytopathogenic fungi and foodborne pathogens on produce". Objective: To screen three LAB (Lactobacillus plantarum, Pediecoccus acidilactici, and Pediococcus pentosaceus) for antimicrobial activity against the foodborne pathogens Listeria monocytogenes, Salmonella, and Escherichia coli. Methods: 1 ml of the pathogen was placed into a petri dish. Then. 15-20 mi molten tryptic soy agar (TSA) tempered to 50°C was added. When the TSA solidified, a loopful of LAB was spotted onto the agar. Plates were incubated at 35°C for 24 hours then the zone of inhibition was measured from the boarder of the bacterial colony to the perimeter of the clkaring. Results: E col OIS7H7 Questions: 1. What is the independent variable? 2. What is the dependent variable? P. pontonv

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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3. Suggest a control for this experiment? 4. What is the optimal temperature range for Listeria monocyogenes? Is L. monocyogenes a psychrophile, psychrophile, mesophile, or themophile? 5. What the optimal pH range for Lactobacillus plantarum? Is Lb. plantarum an acidophile, neutraphile, or alkalophile? 6. What is the toxin produced by E. coli O157:H7 that was encode by phage as a consequence of lysogenic conversion? Explain the medical importance. 7. Which bacterial culture (Lb. plantarum, P. acidilactici, or P. pentosaceus) was most effective against L. monocytogenes and Salmonella? Explain why. 8. What are the limitations of using an agar disk diffusion assay to assess the effectiveness of an antiseptic, disinfectant, or, in this case, a biological control agent on the growth of bacteria of interest? 9. LAB produce organic acids that have shown to be effective against controlling the growth of foodborne pathogens. What specific organic acid is produced by LAB during fermentation? 10. What is the logical next step to validate the efficacy of the biological control agent?
Transcribed Image Text:3. Suggest a control for this experiment? 4. What is the optimal temperature range for Listeria monocyogenes? Is L. monocyogenes a psychrophile, psychrophile, mesophile, or themophile? 5. What the optimal pH range for Lactobacillus plantarum? Is Lb. plantarum an acidophile, neutraphile, or alkalophile? 6. What is the toxin produced by E. coli O157:H7 that was encode by phage as a consequence of lysogenic conversion? Explain the medical importance. 7. Which bacterial culture (Lb. plantarum, P. acidilactici, or P. pentosaceus) was most effective against L. monocytogenes and Salmonella? Explain why. 8. What are the limitations of using an agar disk diffusion assay to assess the effectiveness of an antiseptic, disinfectant, or, in this case, a biological control agent on the growth of bacteria of interest? 9. LAB produce organic acids that have shown to be effective against controlling the growth of foodborne pathogens. What specific organic acid is produced by LAB during fermentation? 10. What is the logical next step to validate the efficacy of the biological control agent?
Experiment #1 From Antoinette de Senna's Master's thesis on "Screening of biological control organisms for the management of phytopathogenic fungi and foodborne pathogens on produce". Objective: To screen three LAB (Lactobacillus plantarum, Pediococcus acidilactici, and Pediococcus pentosaceus) for antimicrobial activity against the foodborne pathogens Listeria monocytogenes, Salmonella, and Escherichia coli. Methods: 1 ml of the pathogen was placed into a petri dish. Then, 15-20 ml molten tryptic soy agar (TSA) tempered to 50°C was added. When the TSA solidified, a loopful of LAB was spotted onto the agar. Plates were incubated at 35°C for 24 hours then the zone of inhibition was measured from the boarder of the bacterial colony to the perimeter of the clearing. Results: Lmonocytogenes Salmonella E col O157-H7 Questions: 1. What is the independent variable? 2. What is the dependent variable? Pacidilactici
Transcribed Image Text:Experiment #1 From Antoinette de Senna's Master's thesis on "Screening of biological control organisms for the management of phytopathogenic fungi and foodborne pathogens on produce". Objective: To screen three LAB (Lactobacillus plantarum, Pediococcus acidilactici, and Pediococcus pentosaceus) for antimicrobial activity against the foodborne pathogens Listeria monocytogenes, Salmonella, and Escherichia coli. Methods: 1 ml of the pathogen was placed into a petri dish. Then, 15-20 ml molten tryptic soy agar (TSA) tempered to 50°C was added. When the TSA solidified, a loopful of LAB was spotted onto the agar. Plates were incubated at 35°C for 24 hours then the zone of inhibition was measured from the boarder of the bacterial colony to the perimeter of the clearing. Results: Lmonocytogenes Salmonella E col O157-H7 Questions: 1. What is the independent variable? 2. What is the dependent variable? Pacidilactici
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