Homework Help is Here – Start Your Trial Now!
Science
Biology
Experiment 1: Does Enzyme Concentration affect Amylase Activity? To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose solution with 70ul of amylase enzyme solution. Each trial used a different concentration of enzyme solution. At 15 second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylase solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction.

Experiment 1: Does Enzyme Concentration affect Amylase Activity? To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose solution with 70ul of amylase enzyme solution. Each trial used a different concentration of enzyme solution. At 15 second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylase solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
What would be the control variables if any and the controls use in this experiment?
**Experiment 1: Does Enzyme Concentration Affect Amylase Activity?** To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose solution with 70 μl of amylase enzyme solution. Each trial used a different concentration of enzyme solution. At 15-second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylose solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction.
Transcribed Image Text:**Experiment 1: Does Enzyme Concentration Affect Amylase Activity?** To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose solution with 70 μl of amylase enzyme solution. Each trial used a different concentration of enzyme solution. At 15-second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylose solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Intelligence
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS