Exercise: The absorbance of known concentration of potassium permanganate (KMnO4, purple color aqueous solution) is obtained by UV-vis spectrophotometer. The results are tabulated: [KMnO4] Absorbance (λ = 510 nm) 0.0000 1.029 × 10 2.058 × 10 3.087 × 10 4.116 x 10 0.000 0.257 0.518 0.771 1.021 Q1) Plot the absorbance vs concentration and obtain the best trendline equation. What is the value of R2? a.0.99953 b.0.99991 c.0.99993 d.0.99985 e.0.99990 Q2) What is the value of slope to the nearest 5 decimal places? a.2483.96505 b. 2483.96499 c.2483.96501 d.2484.00000 e.2483.99999 Q3) What is the concentration at absorbance of 0.62000? a.0.00025 b.0.00030 c.0.00028 d.0.00026 e.0.00027

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UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or  ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.