Exercise 10 & 11: Carbohydrate catabolism in general - primarily looking at fermentation paths in these exercises Exoenzymes & Amylase - only specific enzyme for this exercise Starch agar (what looks like for positive and negative, what enzyme you are testing for, how to do test - must add iodine reagent before reading!) Sugar Fermentation tubes: what testing for (acid or acid/gas production), observe and interpret results completely (A = acid, Ag = Acid + gas, K= alkaline) [Acid = yellow; K= red} MRVP broth - do twice, once for MR methyl red pathway and one for VP test Voges Proskauer pathway How to do both tests, what is each testing for, observe and interpret results completely, what reagents are added MR (mixed acid fermentation) VP (2,3 butanediol fermentation) Citrate slant (citrate sole carbon source) TSI (glucose/lactose/sucrose fermentation to acids, H₂S production) observe and interpret results completely like A/A, K/K, A/H2S, etc. what processes are occurring in butt vs slant for slant sugar reactions - recall, in slant, you cannot distinguish if one or the other sugar present (lactose and or sucrose) has been fermented to acids, only that at least one of them has been fermented to acids
Exercise 10 & 11: Carbohydrate catabolism in general - primarily looking at fermentation paths in these exercises Exoenzymes & Amylase - only specific enzyme for this exercise Starch agar (what looks like for positive and negative, what enzyme you are testing for, how to do test - must add iodine reagent before reading!) Sugar Fermentation tubes: what testing for (acid or acid/gas production), observe and interpret results completely (A = acid, Ag = Acid + gas, K= alkaline) [Acid = yellow; K= red} MRVP broth - do twice, once for MR methyl red pathway and one for VP test Voges Proskauer pathway How to do both tests, what is each testing for, observe and interpret results completely, what reagents are added MR (mixed acid fermentation) VP (2,3 butanediol fermentation) Citrate slant (citrate sole carbon source) TSI (glucose/lactose/sucrose fermentation to acids, H₂S production) observe and interpret results completely like A/A, K/K, A/H2S, etc. what processes are occurring in butt vs slant for slant sugar reactions - recall, in slant, you cannot distinguish if one or the other sugar present (lactose and or sucrose) has been fermented to acids, only that at least one of them has been fermented to acids
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:Exercise 10 & 11:
Carbohydrate catabolism in general - primarily looking at fermentation paths in these exercises
Exoenzymes & Amylase - only specific enzyme for this exercise
Starch agar (what looks like for positive and negative, what enzyme you are testing for, how to do test - must
add iodine reagent before reading!)
Sugar Fermentation tubes:
what testing for (acid or acid/gas production), observe and interpret results completely (A = acid, Ag =
Acid + gas, K= alkaline) [Acid = yellow; K= red}
MRVP broth - do twice, once for MR methyl red pathway and one for VP test Voges Proskauer pathway
How to do both tests, what is each testing for, observe and interpret results completely, what reagents
are added
MR (mixed acid fermentation)
VP (2,3 butanediol fermentation)
Citrate slant (citrate sole carbon source)
TSI (glucose/lactose/sucrose fermentation to acids, H₂S production)
observe and interpret results completely like A/A, K/K, A/H2S, etc.
what processes are occurring in butt vs slant
for slant sugar reactions - recall, in slant, you cannot distinguish if one or the other sugar present
(lactose and or sucrose) has been fermented to acids, only that at least one of them has been
fermented to acids
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