Exactly 5.00 mL aliquots of a solution containing analyte X were transferred into 50.00-mL volumetric flasks and the pH of the solution is adjusted to 9.0. The following volumes of a standard solution containing 2.00 µg/mL of X were then added into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50 and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, and the following values were obtained: 3.26, 4.80, 6.42, 8.02 and 9.56, respectively. ii. Using relevant functions in Excel, derive a least-squares equation for the data, and use the parameters of this equation to find the concentration of the phenobarbital in the unknown solution.

Exactly 5.00 mL aliquots of a solution containing analyte X were transferred into 50.00-mL volumetric flasks and the pH of the solution is adjusted to 9.0. The following volumes of a standard solution containing 2.00 µg/mL of X were then added into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50 and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, and the following values were obtained: 3.26, 4.80, 6.42, 8.02 and 9.56, respectively. ii. Using relevant functions in Excel, derive a least-squares equation for the data, and use the parameters of this equation to find the concentration of the phenobarbital in the unknown solution.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Related questions

Q: The concentration of Nat in the sea water sample was determined with atomic fluorescence…

A: According to Lamber Beer Law A = €cl € = molal absorptivity coefficient C = concenteration l = path…

Q: You have measured the blank for a determination of arsenic in food samples by hydride-generation…

A: Given, Sr. No. (N) 1 2 3 4 5 6 7 8 9 10 Blank values(ppb) 0.23 0.14 0.16 0.28 0.18 0.09 0.10…

Q: Suppose you are analyzing mercury ions (Hg2+) in drinking water samples with atomic absorption…

A: A) Hg^2+ To accurately determine the concentration of mercury ions (Hg2+) in a sample using…

Q: You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml)…

A: First, we have to find the corrected absorbance values by subtracting the absorbance of each value…

Q: A mixture is prepared by combining 10.72 mL of 2.52 x 10-3 M Fe(NO3)3 with 10.00 mL of 1.76 x 10-3 M…

A: Given that: Initial concentration of Fe(NO3)3 is 2.52 x 10-3 M. Volume of Fe(NO3)3 solution is 10.72…

Q: A sample of water from the North Sea was analysed by ion-chromatography and the sulfate…

Q: MF: 3H -3 2H 1H illu DU: PPM 6H

A: Double bond equivalence/ degree of unsaturation = (2C+2-H-X+N)/2where, C is number of carbon atoms…

Q: A spectrophotometer method for the quantitative analysis of Pb2+ in blood uses Cu2+ as an internal…

A: given : SASIS = 0.8320.351= 2.37 CA = concentration of analyte = 1.75 CIS = concentration of…

Q: 1. Why is it recommended to have a carbon monoxide detector on each floor of a home?

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: Determining the sodium concentration in coffee with flame atomic absorption spectroscopy (FAAS) can…

Q: A 0.05 mM solution of DCPIP was placed in a 1 cm spectrophotometer cuvette and a beam of light at…

A: We know that absorbance = log (I0/I) = e*c*l

Q: Assuming an integrated luminosity of 100 fb is collected at the ATLAS detector at the LHC, calculate…

A: The aim of this question is to explain the integrated luminosity of 100 fb(-1) of the ATLAS…

Q: To determine the concentration of these analyte ions in a mixture, the mixture absorbance is…

A: This is application of the Lambert beer law . Here we are required the molar concentration of X3+…

Q: Atomic absorption spectroscopy was used to determine the amount of aluminum in a sample of…

A: The measured blank values are: 0.0073, 0.0082, 0.0110, 0.0101, 0.0102, 0.0098, and 0.0090 AU number…

Q: The Na* concentration in a blood serum sample was determined using the method of standard addition…

Q: DIfference between different types of UV spectrometers based on their advantages and disadvantages

A: Answer - UV spectroscopy or UV–visible spectrophotometry refers to absorption spectroscopy or…

Q: Quinine in a 1.939-g tablet was to be analyzed by dissolving it with 0.10 M HCI to a final volume of…

A: First relate the 2 using the formula: F = 2.3030SϕIo ebC The S and phi (ϕ) is just constants…

Q: Mass spectral measurements were made on 10 replicate low concentration Cd-EDTA samples: 181.5,…

Q: 10 cm 3 air saturated Fricke dosimetry solution in a 1 cm diameter tube is irradiated for 10 min in…

A: Absorbance is a measure of the quantity of light absorbed by a sample. It can be calculated by using…

Q: Lithium in a sample is determined by flame emission spectrometry, using a standard additions…

A: If 10 microlitres of 0.010M of lithium nitrate on addition gives 14.6 units, then the sample without…

Q: The data given below was generated by a student in Bio 240L during a Bradford Assay. A standard…

A: According to the Lambert Beer law when a monochromatic light is passed through a solution, the rate…

Concept explainers

Acid Base Titration

An acid-base titration is the method of quantitative analysis for determining the concentration of an acid and base by exactly neutralizing it with a standard solution of base or acid having known concentration.

Question

Exactly 5.00 mL aliquots of a
solution containing analyte X were
transferred into 50.00-mL
volumetric flasks and the pH of
the solution is adjusted to 9.0. The
following volumes of a standard
solution containing 2.00 µg/mL of
X were then added into each flask
and the mixture was diluted to
volume: 0.000, 0.500, 1.00, 1.50
and 2.00 mL. The fluorescence of
each of these solutions was
measured with a fluorometer, and
the following values were
obtained: 3.26, 4.80, 6.42, 8.02
and 9.56, respectively.
ii. Using relevant functions in
Excel, derive a
least-squares equation for
the data, and use the
parameters of this
equation to find the
concentration of the
phenobarbital in the
unknown solution.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

Step 2

Step 3

Step 4

Step 5

Step 6

Step by step

Solved in 6 steps with 6 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

Determine the mean paracetamol content per tablet (in mg), using the following values: the mean absorbance 0.13, and molar absorption coefficient 9601.9 M−1 cm−1 , where the equivalent half of one tablet mass of powdered paracetamol tablets transferred is 296.4 mg and the mean paracetamol tablet weight is 599.1 mg. Give your answer to TWO decimal places (you do not need to include the units in your answer below).
The students conducted the assay for LDH activity using the two serum samples. Each cuvette (path length 1 cm) contained 3ml of a suitable assay buffer (including pyruvate as substrate). 20 microlitres of either serum was added to the cuvette and the absorbance values immediately recorded at the optimum wavelength for a period of 5 minutes (absorbance readings taken every 30 seconds). Protein concentration of serum sample (mg/ml) Change in absorbance at optimum wavelength per minute Control serum (C) 8 -0.04 Diseased serum (D) 7.8 -0.6 1c. Using the molar absorption coefficient of NADH as 6220 M-1 cm-1, and by application of the Beer-Lambert law, estimate the enzyme activity in the two samples (C and D). Express activity as moles per second. 1d. Estimate the specific activity of the two samples (moles per second per microgram).
13
Analyte in an unknown gave a signal of 9.3 mV. When 100 mL of 0.211 M standard was added to 100.0 mL of unknown, the signal increased to 16.7 mV. Find the concentration of the original unknown solution.