Estimation of the Water Potential (Ψw) of Storage Tissue by Volume Change Method

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Section E. Estimation of the Water Potential (Ψw) of Storage Tissue by Volume Change Method pls answer tysm!
Then
9.
Determine the molar concentration of sucrose
that gives 0% change in weight. Obtain the Ys using
the previous formula.
10. Then compute the water potential using the fomula:
Yw = Ys + Yp
Your answer will be in units of J m (energy per unit
volume) which are equivalent to pascal (Pa). Convert
to MPa by dividing to 10°.
What is the sucrose concentration at which zero percent
change in weight is observed?
What is the water potential Yw of Tradescantia or Allium
сера сells?
What is the water potential Yw of the Solanum tuberosum
potato cells?
Transcribed Image Text:Then 9. Determine the molar concentration of sucrose that gives 0% change in weight. Obtain the Ys using the previous formula. 10. Then compute the water potential using the fomula: Yw = Ys + Yp Your answer will be in units of J m (energy per unit volume) which are equivalent to pascal (Pa). Convert to MPa by dividing to 10°. What is the sucrose concentration at which zero percent change in weight is observed? What is the water potential Yw of Tradescantia or Allium сера сells? What is the water potential Yw of the Solanum tuberosum potato cells?
A similar experiment is as follows:
1.
Obtain 11 (50-ml capacity) beakers and mark
from 1-11.
In each beaker add 20 ml of one concentration of
the following sucrose solutions: Distilled water, 0.1.,
0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 M
3.
2.
Using a cork borer, cut 55 cylinders from a large
potato or any storage tissue and trim each cylinder to
0.5 cm in length.
Place the cylinders in a covered beaker as you
cut them to prevent them from drying up.
Divide the cylinders in sets of 5. Weigh each set
(to 0.01 g). Put one set of cylinders in each of the
beakers.
4.
5.
Record the fresh weights of each set in
Table 6.
After 90 minutes, remove the cylinders, gently
blot dry with paper towels and reweigh.
7.
6.
Subtract the initial weights from the final weights.
Divide the difference by the initial weight and multiply
by 100 to get the % weight change. Complete Table 6.
8.
Plot the% change in weight (y, ordinate) vs.
sucrose concentration (x, abscissa) on a graph. Draw
the best fit straight line through the points.
Table 6a.
Sucrose
Concentration
0 0.1 0.2 0.3 0.4 0.5 0.6 | 0.7 0.8 0.9 1.0
(Molarity)
3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.0
Initial Weight (g)
3.61 3.38 3.103.02 2.93 2.70 2.50 1.90 1.80 1.63 1.38
Final Weight (g)
A Weight (g)
% Weight Change
Table 6b.
Sucrose
Concentration
0 0.1 0.2 0.3 0.4 0.5 0.6
0.8 0.9 1.0
(Molarity)
5.00 5.00 5.005.00 5.00 5.00 5.00 5.00 5.00 5.00 5.0
Initial Weight (g)
6.41 6.38 5.80 5.054.50 4.00 3.50 2.90 2.20 1.75 1.38
Final Weight (g)
A Weight (g)
% Weight
Change
Transcribed Image Text:A similar experiment is as follows: 1. Obtain 11 (50-ml capacity) beakers and mark from 1-11. In each beaker add 20 ml of one concentration of the following sucrose solutions: Distilled water, 0.1., 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 M 3. 2. Using a cork borer, cut 55 cylinders from a large potato or any storage tissue and trim each cylinder to 0.5 cm in length. Place the cylinders in a covered beaker as you cut them to prevent them from drying up. Divide the cylinders in sets of 5. Weigh each set (to 0.01 g). Put one set of cylinders in each of the beakers. 4. 5. Record the fresh weights of each set in Table 6. After 90 minutes, remove the cylinders, gently blot dry with paper towels and reweigh. 7. 6. Subtract the initial weights from the final weights. Divide the difference by the initial weight and multiply by 100 to get the % weight change. Complete Table 6. 8. Plot the% change in weight (y, ordinate) vs. sucrose concentration (x, abscissa) on a graph. Draw the best fit straight line through the points. Table 6a. Sucrose Concentration 0 0.1 0.2 0.3 0.4 0.5 0.6 | 0.7 0.8 0.9 1.0 (Molarity) 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.0 Initial Weight (g) 3.61 3.38 3.103.02 2.93 2.70 2.50 1.90 1.80 1.63 1.38 Final Weight (g) A Weight (g) % Weight Change Table 6b. Sucrose Concentration 0 0.1 0.2 0.3 0.4 0.5 0.6 0.8 0.9 1.0 (Molarity) 5.00 5.00 5.005.00 5.00 5.00 5.00 5.00 5.00 5.00 5.0 Initial Weight (g) 6.41 6.38 5.80 5.054.50 4.00 3.50 2.90 2.20 1.75 1.38 Final Weight (g) A Weight (g) % Weight Change
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