Erwin Chargaff discovered that although the nucleotide composition of DNA varies from one species to another, that composition always follows certain rules. While variation in nucleotides helps to explain the complexity of life, the physical structure of the DNA itself can also help certain organisms adapt to particular environments. Imagine you are working with a research team sampling the ocean floor near a hot vent that releases a steady stream of hot water. The hot water has a temperature of about 45°C, while the surrounding ocean has a temperature of 6°C. Nucleotide Presence in DNA of bacterial Presence in DNA of bacterial sample 1 (percent) sample 2 (percent) adenine 21 36 cytosine guanine thymine Your team collects two samples of bacteria - one from the mouth of the hot vent and one from the ocean floor about 20 m away. When you return to the lab, you isolate the DNA from these bacteria to determine their nucleotide composition. The table shows the results of your test for the adenine content of the DNA.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
|
Presence in DNA of bacterial sample 1 (percent) |
Presence in DNA of bacterial sample 2 (percent) |
Adenine |
31 |
18 |
Cytosine |
19 |
32 |
Guanine |
19 |
32 |
Thymine |
31 |
18 |
I already did the chart I just need help with the question please
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