Enzyme reactions Example The reaction between nicotineamide mononucleotide and ATP to form nicotineamide-adenine dinucleotide and pyrophosphate is catalyzed by the enzyme nicotinamide mononucleotide adenylyl transferase. The following table provides typical data obtained at a pH of 4.95. The substrate, S, is nicotinamide mononucleotide and the initial rate, v, is the μmol of nicotinamide-adenine dinucleotide formed in a 3-min reaction period. [S] (mM) 0.138 0.220 0.291 0.560 0.766 1.460 v (umol) 0.148 0.171 0.234 0.324 0.390 0.493 Determine values for Vax and Km.

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Enzyme reactions
Example
The reaction between nicotineamide mononucleotide and ATP to form
nicotineamide-adenine dinucleotide and pyrophosphate is catalyzed by the
enzyme nicotinamide mononucleotide adenylyl transferase. The following
table provides typical data obtained at a pH of 4.95. The substrate, S, is
nicotinamide mononucleotide and the initial rate, v, is the μmol of
nicotinamide-adenine dinucleotide formed in a 3-min reaction period.
[S] (mM) 0.138 0.220 0.291
0.560 0.766 1.460
v (umol)
0.148 0.171
0.234
0.324 0.390 0.493
Determine values for Vax and Km.
Transcribed Image Text:Enzyme reactions Example The reaction between nicotineamide mononucleotide and ATP to form nicotineamide-adenine dinucleotide and pyrophosphate is catalyzed by the enzyme nicotinamide mononucleotide adenylyl transferase. The following table provides typical data obtained at a pH of 4.95. The substrate, S, is nicotinamide mononucleotide and the initial rate, v, is the μmol of nicotinamide-adenine dinucleotide formed in a 3-min reaction period. [S] (mM) 0.138 0.220 0.291 0.560 0.766 1.460 v (umol) 0.148 0.171 0.234 0.324 0.390 0.493 Determine values for Vax and Km.
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