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- Provide a detailed description and hand drawn figure of each of the following. (1) Mismatch Repair (2) Nucleotide-Excision Repair10. The three components of a plasmid which are crucial for gene cloning purpose are: 1) 2) 3)1. TRUE OR FALSE: a) Okazaki fragments are short DNA pieces that explain how the DNA polymerase can continue the synthesis of the new strand. b) Okazaki fragments are short DNA pieces that explain how the DNA polymerase can continue the synthesis of the new strand.
- 1 (a) What do ApR, TcR, and ori on the pBR322 map represent and discuss there individual functions? (b)Does the undigested plasmid show more than a single band when electrophoresed? Why? (c)What other kinds of molecules, in addition to plasmid DNA would you expect to find in a sample of plasmid DNA extraction? (This is not a graded assignment)Isolation of DNA is a crucial step for genetic engineering. (i) What is the difference between genomic DNA and plasmid DNA? (ii) Describe the common procedure for isolating genomic DNA without using the DNA extraction kit. ..1..A.How could endonucleases interfere with the transformation procedure? B. Does supercoiled or nicked plasmid get transformed more efficiently? Why?
- Some Steps Involved in Creating Recombinant DNA 1. Plasmid DNA is cut at the restriction site.2. Foreign DNA is cut at the restriction site.3. Plasmid DNA is opened and sticky ends are formed.4. Foreign DNA restriction fragments are isolated5. Target sequence in the plasmid is recognized by the restriction endonuclease.6. Target sequences in the foreign DNA are recognized by the restriction endonuclease.7. Foreign DNA restriction fragment is inserted into the plasmid at the restriction site.8. DNA ligase splices the foreign restriction fragment and the plasmid together. Identify the correct sequence of steps involved in cutting and reassembling DNA molecules to make recombinant DNA. Start with preparation of the plasmid DNA. Select one: a. 6, 2, 4, 7, 8, 5, 1, 3 b. 5, 1, 3, 6, 2, 4, 8, 7 c. 5, 1, 3, 6, 2, 4, 7, 8 d. 6, 2, 4, 5, 1, 3, 8, 739. Which of the following are general features found in a plasmid used for bacterial cloning? Group of answer choices Origin of replication Multiple cloning site Multiple cloning site and antibiotic resistance gene Multiple cloning site, antibiotic resistance gene, and origin of replication Locus of transformation Locus of transformation, multiple cloning site, and antibiotic resistance gene Antibiotic resistance genePlasmids are very important for recombinant DNA work. 1.) Describe the components that are important to a cloning vector.
- 35) A researcher inserted DNA fragments from an organism into expression vector plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be an effective means of identifying which clones contain a specific gene of interest?24. In recombinant DNA technology, genes are usually inserted into what form of DNA before being transferred into bacteria? - [ ] Teddy bear - [ ] Nucleus - [ ] Transposon - [ ] Ribosome - [ ] Plasmid 25. In PCR , what is happening at the denaturation step (94 )? - [ ] RNA polymerase creates a new RNA strand . - [ ] DNA strands come apart . - [ ] DNA polymerase creates a new DNA strand. - [ ] Primers bind to the target DNA. - [ ] Nucleotides bind to the primer .Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.