elect molecular outcomes that can be achieved with the CRISPR-Cas9 system (Check all that apply.) Gene activation Gene repression Loss of gene function Creation of a targeted mutation within a gene Creation of many copies of a specific gene
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elect molecular outcomes that can be achieved with the CRISPR-Cas9 system (Check all that apply.)
-
Gene activation
-
Gene repression
-
Loss of gene function
-
Creation of a targeted mutation within a gene
-
Creation of many copies of a specific gene
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- Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant sickle cell anemia allele of beta globin. This mutation destroys an MstII restriction site normally present in the beta globin gene. This difference between the normal allele and the mutant allele can be detected with Southern blotting. Using a labeled beta globin gene as a probe, what differences would you expect to see for a Southern blot of the normal beta globin gene and the mutant sickle cell gene?Describe the primary procedure(key steps no details) that you will follow to clone the C-peptide gene from the RNA above into the only vector you have, a pUC expression vector cut open usingEcoR1which has a 5¢-recognition site and a 3¢- Hind III in the MCS?BIOLOGICAL IMPLICATIONS OF CRISPR-CAS9
- Anti apoptosome anti N anti protein X anti caspase based on thé data above where is the WT protein actually cleaved D380 D380 and D835 D835 D835 and D840 D840 all are cleaved none are cleaved D380 and D840 if you compared the size of WT and the D380E mutant before apoptosis on a western blot how would they appear? D380E would be larger they would be indistinguishable WT would be larger how many amino acids long is the larger band in the lane labeled D380E/D835EEnhancer sequences may be very far from the genes they affect but they are always upstream of the gene sequence. True FalseYou are working for a pharmaceutical company are are tasked with creating E. Coli that express an influenza antigen (a protein that is recognized by our immune system) fortreatment of the influenza virus. The cDNA of the antigen is available in a carrier vector(pCARRY) that contains a kanamycin resistance gene as shown on the left below. Inorder to get E. Coli to express the antigen, you need to clone the gene for the influenzaantigen into an expression vector that contains an E. Coli-specific promoter. You obtain such an expression vector, which contains an ampicillin resistance gene as shown on the right below (pBACTERIA) a. An end generated by digestion with BamH1 can be ligated to an end generated by digestion with BlgII. Why is this possible? b. To clone the gene for the influenza antigen from pCARRY into pBACTERIA plasmid:i. What restriction enzyme should you use to digest pCARRY? ii. What restriction enzyme should you use to digest pBACTERIA? c. After you ligate the products of…
- Noncoding ribonucleic acid (RNA) interference/regulation in reguards to gene expression. Provide examples.During a CRISPR-Cas9 exp., yeast are transformed with PML104-9RNA1 plasmid and HDR1 template to edit ADE2 gene. Then answer the following questions.. 1.In yeast, what is the template from which the GRNA is transcribed? 2. The HDR fragment must enter the homology directed repair. 3. The Cas9 gene is transcribed from transported from 4. what happens after translation of Cas9? of yeast cell to be used for as template, transcript is of the celi, and then protein is translated. to1. You are investigating a protein that has the amino acid sequence N ... Ala – Thr - Asn – Trp – Lys - Arg - Gly – Phe – Thr ... C within its primary structure. You found that several of the mutations affecting this protein produced shortened protein molecules that terminated within this region. In one of the mutants, the Asn became the terminal (last) amino acid. (a) What DNA single-base changes(s) would cause the protein to terminate at the Asn residue? (b) What other potential sites do you see in the DNA sequence encoding this protein where mutation of a single base pair would cause premature termination of translation? >
- both noncoding and coding). a. Which types of SNPs affect protein production or function for the gene of interest? b. Which types of SNPs might be identified in a GWAS? Biolnteractive.org (including Updated November 20% Page 1 ofMatching Match each item with the correct statement below. Not all terms will be used a. 5' GTP cap f. RFLPs b. Target copy g. sticky ends c. Ligase h. cloned DNA d. Cas-9 i recombinant DNA j. ampicillin e. Host cell 1. A cell (usually a bacteria) that has a gene put in it so the gene can be cloned 2. An example of a post-transcriptional modification of mRNA 3. Sections of DNA that vary between individuals. These can be used for DNA fingerprinting 4. May be used following transformation to kill off cells that a gene didn't enter 5. Molecule of DNA created during PCR that is the desired length 6. A molecule of DNA that comes from two different sources that are spliced together 7. Ends of DNA cut by a restriction enzyme where not all nucleotides are paired (i.e. ends are uneven) 8. Protein that works with CRISPR to cut DNA at a specific spot5 5 S 6 5 5 5 6 U 6 U 6 5:14 PM | 0.2KB/s HHHHH R R U RUUR ARU AP AP R U U R R AP R R R AP MOLECULAR...GENETICS. Describe gene regulation at transcription level. Explain the role of antsense RNA in control mechanism. Describe translational control mechanisms. Describe common DNA damages. Distinguish excision and mismatch repair. Describe the role of recA protein in recombination repair Elaborate on SOS repair mechanism. Define thymine dimer. How are they formed and repaired? Describe the molecular basis of mutation. 11 Leu+ Met+ Arg+ Write a detailed note on spontaneous mutation. Explain about mutant detection methods. Define reverse mutation. Describe the mechanism underlying Intragenic and intergenic suppressor mutations Describe the transposition mechanisms. 13 Vo LTE UNIT IV Time (Min) Describe the process of generalised transformation occurring in bacterial chromosome and plasmid. Elaborate on molecular mechanism and significance of transformation 22 Describe the process of…
