E. coli is grown in a chemostat with a working volume of 1.0 litre. A medium containing 4 g litre¹ of glucose as growth limiting substrate is fed to the fermenter at a constant flow rate of 0.5 litre h*¹. The glucose concentration in the outlet stream is 0.2 g litre-1. The cell yield (Yxs) is 0.42 g g¹¹. Determine the cell concentration in the output stream and the specific growth rate. [Ans: x= 1.6 g L¹; μ = 0.5 h¹]

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
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1. E. coli is grown in a chemostat with a working volume of 1.0 litre. A medium containing 4 g litre¹ of
glucose as growth limiting substrate is fed to the fermenter at a constant flow rate of 0.5 litre h¹¹. The
glucose concentration in the outlet stream is 0.2 g litre-1. The cell yield (Yxs) is 0.42 g g¹. Determine
the cell concentration in the output stream and the specific growth rate.
[Ans: x= 1.6 g L¹; μ = 0.5h¹]
Transcribed Image Text:1. E. coli is grown in a chemostat with a working volume of 1.0 litre. A medium containing 4 g litre¹ of glucose as growth limiting substrate is fed to the fermenter at a constant flow rate of 0.5 litre h¹¹. The glucose concentration in the outlet stream is 0.2 g litre-1. The cell yield (Yxs) is 0.42 g g¹. Determine the cell concentration in the output stream and the specific growth rate. [Ans: x= 1.6 g L¹; μ = 0.5h¹]
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