Disease Z is marked by the accumulation of Metabolite M - a product of the reaction: L→M. This reaction is catalyzed by Enzyme B. So to prevent further production of Metabolite M, Enzyme B was tagged as a drug target. To arrest Enzyme B, candidate drug Y was investigated. The kinetics of Enzyme B with and without Drug Y yielded the following results: [S], mM 2 V without Drug Y, mM/s 2.36 V with Drug Y, mM/s 1.42 2.66 3.74 4 3.53 5.71 8.49 10.82 6 8 4.69 10 5.54 A. Determine the Km and Vmax of the enzyme. B. Determine the Km and Vmax of the enzyme with Drug Y. C. What type of inhibition is demonstrated by Drug Y (if any)?
Disease Z is marked by the accumulation of Metabolite M - a product of the reaction: L→M. This reaction is catalyzed by Enzyme B. So to prevent further production of Metabolite M, Enzyme B was tagged as a drug target. To arrest Enzyme B, candidate drug Y was investigated. The kinetics of Enzyme B with and without Drug Y yielded the following results: [S], mM 2 V without Drug Y, mM/s 2.36 V with Drug Y, mM/s 1.42 2.66 3.74 4 3.53 5.71 8.49 10.82 6 8 4.69 10 5.54 A. Determine the Km and Vmax of the enzyme. B. Determine the Km and Vmax of the enzyme with Drug Y. C. What type of inhibition is demonstrated by Drug Y (if any)?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![Disease Z is marked by the accumulation of Metabolite M - a product of the reaction:
L→M. This reaction is catalyzed by Enzyme B. So to prevent further production of
Metabolite M, Enzyme B was tagged as a drug target. To arrest Enzyme B, candidate
drug Y was investigated. The kinetics of Enzyme B with and without Drug Y yielded the
following results:
[S], mM
2
V without Drug Y, mM/s
2.36
V with Drug Y, mM/s
1.42
2.66
3.74
4
3.53
5.71
8.49
10.82
6
8
4.69
10
5.54
A. Determine the Km and Vmax of the enzyme.
B. Determine the Km and Vmax of the enzyme with Drug Y.
C. What type of inhibition is demonstrated by Drug Y (if any)?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F10b748db-6346-46d1-af4f-376595693c91%2F3578336d-815f-4ba5-96b4-589a82e2fb0e%2Foqudrw_processed.png&w=3840&q=75)
Transcribed Image Text:Disease Z is marked by the accumulation of Metabolite M - a product of the reaction:
L→M. This reaction is catalyzed by Enzyme B. So to prevent further production of
Metabolite M, Enzyme B was tagged as a drug target. To arrest Enzyme B, candidate
drug Y was investigated. The kinetics of Enzyme B with and without Drug Y yielded the
following results:
[S], mM
2
V without Drug Y, mM/s
2.36
V with Drug Y, mM/s
1.42
2.66
3.74
4
3.53
5.71
8.49
10.82
6
8
4.69
10
5.54
A. Determine the Km and Vmax of the enzyme.
B. Determine the Km and Vmax of the enzyme with Drug Y.
C. What type of inhibition is demonstrated by Drug Y (if any)?
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