change the linking number (Lk) by 1 or a multiple of 1cleave both DNA strandsAnswer Bankcouples energy from ATP hydrolysis to the introduction of negative supercoilscleave one strand of DNAregulate DNA supercoilingchange the linking number (Lk) by a multiple of 2 Determine whether the phrases describe type I topoisomerases, type II topoisomerases, or both type I and typeII topoisomerases.Type I TopoisomerasesType II topoisomerasesBoth

These are enzymes that catalyse temporary breaks in the circular DNA molecule to wind or unwind DNA.…

Answered: Determine whether the phrases describe…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

PRDM9 recruits SPO11, a topoisomerase type II like protein, to induce DNA double strand breaks. Illustrate Illustrate and explain and explain how SPO11 releases SPO11-linked oligonucleotides.
A conditional mutation expresses its mutant phenotype only under certain conditions (the restrictive conditions) and expresses the normal phenotype under other conditions (the permissive conditions). One type of conditional mutation is a temperature-sensitive mutation, which expresses the mutant phenotype only at certain temperatures. Strains of E. coli have been isolated that contain temperature-sensitive mutations in genes encoding different components of the replication machinery. In each of these strains, the protein produced by the mutated gene is nonfunctional under the restrictive conditions. You grow these strains under the permissive conditions and then abruptly switch them to the restrictive conditions. After one round of replication under therestrictive conditions, you isolate DNA from each strain and analyze it. What characteristics would you expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes the following…
An archaeon (Sulfolobus acidocaldarius) found in acidic hot springs contains a topoisomerase that catalyzes the ATP-driven introduction of positive supercoils into DNA. How might this enzyme be advantageous to this unusual organism?
When Avery and his colleagues had obtained what was concluded to be the transforming factor from the IIIS virulent cells, they treated the fraction with proteases, RNase, and DNase, followed in each case by the assay for retention or loss of transforming ability. What were the purpose and results of these experiments? What conclusions were drawn?
In transformation, a bacterial cell takes up DNA fragments from its surroundings. The figure shows the process of natural transformation. Position the labels below with the correct images. (A) Donor and recipient genomes The donor strand is integrated into the recipient chromosome and the displaced bacterial DNA is degraded. A wild-type donor cell is in the vicinity of a double auxotroph. If the wild-type cell lyses, it releases DNA into the environment. One cell is identical to the original recipient, while the other carries the wild-type genes. A competent recipient cells a receptor site on its surface. One strand of donor DNA enters the cell and pairs with a homologous region of the bacterial chromosome The remaining DNA strand is degraded. A donor DNA strand binds to the receptor site. Reset trpC1 Wild-type donor cell Mechanism of natural transformation Donor DNA hal Receptor site- trpc/his double auxotroph Recipient cell Competent cell recipient -Bacterial chromosome (had.bpC") Ma…
A conditional mutation expresses its mutant phenotype only under certain conditions (the restrictive conditions) and expresses the normal phenotype under other conditions (the permissive conditions). One type of conditional mutation is a temperature-sensitive mutation, which expresses the mutant phenotype only at certain temperatures. Strains of E. coli have been isolated that contain temperature-sensitive mutations in genes encoding different components of the replication machinery. In each of these strains, the protein produced by the mutated gene is nonfunctional under the restrictive conditions. You grow these strains under the permissive conditions and then abruptly switch them to the restrictive conditions. After one round of replication under therestrictive conditions, you isolate DNA from each strain and analyze it. What characteristics would you expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes the following…
Does transversion occur, If a purine replaces a pyrimidine, or vice versa ?
Identify the most mistaken (wrong) choice: a) Transcription machinery and an enhancer can bind to the chromosome at the same time. b) Organic matters may interfere with heat treatment of bacterial growth control. ( c) Nitrocellulose can be used to filter out microorganisms from a liquid solution. d) Time to kill a bacterial culture is not proportional to the number of microbes in the culture
DNA unwinding is done bya) Ligaseb) Helicasec) Topoisomerased) Hexonuclease
Telomerase activity has been found to be 10 to 20 times more active in cancer cells than in normal somatic cells. What is the significance of this circumstance?
A 19 year old female patient is diagnosed with chronic myelogenous leukemia. Karyotype analysis shows that the leukemic cells of this patient are heterozygous for a reciprocal translocation involving chromosomes 9 and 22. However, none of the normal, nonleukemic cells of this patient contain the translocation. a) Describe a molecular test to determine if chemotherapy given to the patient described would be completely succesful. (That is, devise a method to make sure that the patient's blood would be free of leukemic cells.) Be as specific as possible.

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS

Determine whether the phrases describe type I topoisomerases, type II topoisomerases, or both type I and type II topoisomerases.