change the linking number (Lk) by 1 or a multiple of 1cleave both DNA strandsAnswer Bankcouples energy from ATP hydrolysis to the introduction of negative supercoilscleave one strand of DNAregulate DNA supercoilingchange the linking number (Lk) by a multiple of 2 Determine whether the phrases describe type I topoisomerases, type II topoisomerases, or both type I and typeII topoisomerases.Type I TopoisomerasesType II topoisomerasesBoth

These are enzymes that catalyse temporary breaks in the circular DNA molecule to wind or unwind DNA.…

Answered: Determine whether the phrases describe…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Using a named example, discuss the advantages of using E. coli to produce recombinant proteins.
PRDM9 recruits SPO11, a topoisomerase type II like protein, to induce DNA double strand breaks. Illustrate Illustrate and explain and explain how SPO11 releases SPO11-linked oligonucleotides.
Describe how helicase, topoisomerase, and single-strand binding protein are important for the unwinding of the DNA double helix.
An archaeon (Sulfolobus acidocaldarius) found in acidic hot springs contains a topoisomerase that catalyzes the ATP-driven introduction of positive supercoils into DNA. How might this enzyme be advantageous to this unusual organism?
Describe three examples of specific recombinases that have been discussed in this class. Include a brief description for the function of each specific recombinase.
A number of scientists who study cancer treatment have become interested in telomerase. Why? How might anticancer therapies that target telomerase work?
In transformation, a bacterial cell takes up DNA fragments from its surroundings. The figure shows the process of natural transformation. Position the labels below with the correct images. (A) Donor and recipient genomes The donor strand is integrated into the recipient chromosome and the displaced bacterial DNA is degraded. A wild-type donor cell is in the vicinity of a double auxotroph. If the wild-type cell lyses, it releases DNA into the environment. One cell is identical to the original recipient, while the other carries the wild-type genes. A competent recipient cells a receptor site on its surface. One strand of donor DNA enters the cell and pairs with a homologous region of the bacterial chromosome The remaining DNA strand is degraded. A donor DNA strand binds to the receptor site. Reset trpC1 Wild-type donor cell Mechanism of natural transformation Donor DNA hal Receptor site- trpc/his double auxotroph Recipient cell Competent cell recipient -Bacterial chromosome (had.bpC") Ma…
The following table lists 4 bacterial strains that are partial diploids for lac operon genes. Given the activity of beta-galactosidase measured for each strain in the absence (-lac) or presence (+lac) of lactose, complete the table by choosing the appropriate symbol (+, -, C, S) to indicate the allele of the gene or site missing from the table (blue numbers). strain A BC 5 C D 7 chromosome I O 1 2 4 1 [Select] 9 3 [Select] [Select] [Select] 9 [Select] + + Z + + 6 + I +5 + 10 plasmid O 3 + 7 C Z + 8 8 2 [Select] 4 [Select] 6 [Select] B-gal act. -lac +lac 0.002 0.003 0.002 0.058 0.063 0.121 0.059 0.062 Select] 1 ✔ [ Select] + is C S
what are the types of topoisomerase ?

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Determine whether the phrases describe type I topoisomerases, type II topoisomerases, or both type I and type II topoisomerases.