Design a SIP experiment that would allow you to determinewhich organisms in a lake water sample were capable ofoxidizing the hydrocarbon hexane (C6H14). Assume that fourdifferent species could do this. How would you combineSIP with other molecular analyses to identify thesefour species?
Design a SIP experiment that would allow you to determine
which organisms in a lake water sample were capable of
oxidizing the hydrocarbon hexane (C6H14). Assume that four
different species could do this. How would you combine
SIP with other molecular analyses to identify these
four species?
Stable isotope probing (SIP) uses compounds labelled with specific isotopes for identification of microorganisms metabolizing those compound SIP uses heavy stable isotopes (13C and 15N) to identify metabolically active microbial cells.
Microautoradiography (MAR) is a microscopic technique measures microbial activity using radioisotopes. Fluorescence in situ hybridization (FISH) uses fluorescent nucleic acid probe to identify microorganisms in the environment.
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